Alditols, acetates, chromatography

Fox, A. Krahmer, M. Harrelson, D. Monitoring muramic acid in air (after alditol acetate derivatization) using a gas chromatography-ion trap tandem mass spectrometer. J. Microbiol. Meth. 1996, 27,129-138. 

Englyst, H.N. and Cummings, J.H. (1984) Simplified method for the measurement of total non-starch polysaccharides by gas-liquid chromatography of constituent sugars as alditol acetates. Analyst 109, 937-942. 

Steinberg, R, and Fox, A. (1999), Automated derivatization instrument Preparation of alditol acetates for analysis of bacterial carbohydrates using gas chromatography-mass spectrometry, Anal. Chem.,11,1914-1917. 

The method was used in studies of a fungal heterogalactan.150 The polysaccharide was subjected to successive tritylation, methylation, detritylation, p-toluenesulfonylation, reaction with sodium iodide, and, finally, reaction with sodium p-toluenesulfinate. The product was then treated with sodium methylsulfinyl carbanion in dimethyl sulfoxide, the product remethylated, and the polysaccharide material recovered by gel chromatography. The polymer was hydrolyzed, and the sugars in the hydrolyzate were analyzed, as the alditol acetates, by g.l.c.-m.s.1 The analysis revealed that —60% of the hexose residues that were unsubstituted at C-6 had been eliminated. As the product was still polymeric, it was concluded that these residues had constituted a part of side chains linked to a main chain of (1 — 6)-linked D-galactose residues. 

E3.2 Determination of Neutral Sugars by Gas Chromatography of Their Alditol Acetates... 

Determination of the monosaccharide composition by gas chromatography of alditol acetates is commonly used for cell wall analyses because the procedure gives a single peak for each sugar. An alternative is to prepare trimethylsilyl (TMS) derivatives. However, these are easily hydrolyzed by moisture in the atmosphere and therefore should be analyzed in the gas chromatograph immediately, whereas alditol acetates are relatively more stable and could be rerun on the gas chromatograph the following day if required. 

Henry, R.J., Harris, P.J., Blakeney, A.B., and Stone, B.A. 1983. Separation of alditol acetates from plasticisers and other contaminants by capillary gas chromatography. J. Chmmatogr. 262 249-256. 

Gas-liquid chromatography (GLC) alditol acetates, neutral sugar determination, 721 -732 cholesterol measurement, 453-456, 460-462 (tables)... 

Preliminary examinations of dextran structures were conducted by optical rotation, infrared spectroscopy and periodate-oxidation reactions. More detailed results can be achieved by methylation analysis [19]. The hydroxyl groups are methylated with methyl iodide after activation with sodium methylsulfinyl carbanion (Fig. 2). The methyl dextran is hydrolysed to the corresponding different methylated monosaccharides, which are furthermore reduced and peracetylated. The resulting alditol acetates of methylated sugars are separated by gas chromatography and identified by their retention times. In particular, a combined capillary gas-liquid chromatography/mass... 

E. Lau and A. Bacic, Capillary gas chromatography of partially methylated alditol acetates on a high-polarity, cross-linked, fused-silica BPX 70 column,./. Chromatogr., 637 (1993) 100-103. 

Whiton, R. S., Lau, P., Morgan, S. L., Gilbart, J., and Fox, A. (1985). Modifications in the alditol acetate method for analysis of muramic acid and other neutral and amino sugars by capillary gas chromatography-mass spectrometry with selected ion monitoring. /. Chromatogr. A 347, 109—120. 

Adsorption surface, SFC, solvent Interactions, 1>>9 Air monitors. Industrial chromatography, 200 Alditol acetates, chromatogram, 32f Alkyl groups, bonded, HPLC development, 82,83 Amides, enantiomeric, separation, >l3,>l>lf Amine compounds, RPLC, 8>l Amino acids HPLC, 86,87f Ion exchange, 81 ligand exchange, >>... 

Methylation is the most widely used method to determine the position of the glycosyl linkages.Permethylation of glycolipids can be done according to the methods of Hakomori (32) and Ciucanu and Kerek (33). The permethylated derivatives are hydrolyzed and the products converted into partially methylated alditol acetates and identified by gas chromatography-mass spectrometry ( CG-MS) (34). 

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