Alditols chromatography

Reddy, G. P. and Bush, C. A., High-performance anion exchange chromatography of neutral milk oligosaccharides and oligosaccharide alditols derived from mucin glycoprotein, Anal. Biochem., 198, 278, 1991. 

Fox, A. Krahmer, M. Harrelson, D. Monitoring muramic acid in air (after alditol acetate derivatization) using a gas chromatography-ion trap tandem mass spectrometer. J. Microbiol. Meth. 1996, 27,129-138. 

Englyst, H.N. and Cummings, J.H. (1984) Simplified method for the measurement of total non-starch polysaccharides by gas-liquid chromatography of constituent sugars as alditol acetates. Analyst 109, 937-942. 

Certain synthetic heptitols that could not be separated by paper chromatography or by electrophoresis have been separated as their O-trimethylsilyl derivatives.404 The procedure of reductive, alkaline hydrolysis, much used in glycopeptide research, yields alditols that have been estimated as their trimethylsilyl ethers,59,405 and D-glucitol in mammalian nerve has similarly been determined.406... 

Agarose, gels, in chromatography, 14 Albumin, carbohydrate linkage in, 420, 444, 445 Alditols... 

Steinberg, R, and Fox, A. (1999), Automated derivatization instrument Preparation of alditol acetates for analysis of bacterial carbohydrates using gas chromatography-mass spectrometry, Anal. Chem.,11,1914-1917. 

The method was used in studies of a fungal heterogalactan.150 The polysaccharide was subjected to successive tritylation, methylation, detritylation, p-toluenesulfonylation, reaction with sodium iodide, and, finally, reaction with sodium p-toluenesulfinate. The product was then treated with sodium methylsulfinyl carbanion in dimethyl sulfoxide, the product remethylated, and the polysaccharide material recovered by gel chromatography. The polymer was hydrolyzed, and the sugars in the hydrolyzate were analyzed, as the alditol acetates, by g.l.c.-m.s.1 The analysis revealed that —60% of the hexose residues that were unsubstituted at C-6 had been eliminated. As the product was still polymeric, it was concluded that these residues had constituted a part of side chains linked to a main chain of (1 — 6)-linked D-galactose residues. 

An important approach that reduces the number of peaks in the chromatogram involves reduction of sugars to corresponding alditols (Equation 9.10). The alditols are converted to TMS or TFA derivatives prior to chromatography although acetate deriva-... 

Although the use of aldonitrile esters results in reduction of the number of peaks found in GLC without loss of information which occurs when derivatized alditols are used, the chromatography is inefficient. It is to be expected that new chromatographic techniques will be worked out to improve this situation and that they may utilize better column as well as improved deri-vatization procedures. 

E3.2 Determination of Neutral Sugars by Gas Chromatography of Their Alditol Acetates... 

Determination of the monosaccharide composition by gas chromatography of alditol acetates is commonly used for cell wall analyses because the procedure gives a single peak for each sugar. An alternative is to prepare trimethylsilyl (TMS) derivatives. However, these are easily hydrolyzed by moisture in the atmosphere and therefore should be analyzed in the gas chromatograph immediately, whereas alditol acetates are relatively more stable and could be rerun on the gas chromatograph the following day if required. 

Gently evaporate the DCM completely in a stream of instrument-grade air or nitrogen gas, and add 2 ml of DCM. Proceed to gas chromatography of the fully acetylated alditols. 

Henry, R.J., Harris, P.J., Blakeney, A.B., and Stone, B.A. 1983. Separation of alditol acetates from plasticisers and other contaminants by capillary gas chromatography. J. Chmmatogr. 262 249-256. 

Gas-liquid chromatography (GLC) alditol acetates, neutral sugar determination, 721 -732 cholesterol measurement, 453-456, 460-462 (tables)... 

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