Alditol oligosaccharides analysis

In a study completed during the early development of f.a.b.-m.s., both f.d. and f.a.b. were used to characterize 101 fractions containing neutral oligosaccharides isolated from human milk. Samples were examined as their peracetylated alditols. In subsequent work, the structures of two minor acidic oligosaccharides from human milk were investigated. The per-methylated derivatives were analyzed by f.a.b.-m.s., and their compositions and sequences were defined by the f.a.b. data. Methylation analysis and partial formolysis were the other principal methods used. 

Oligosaccharides were isolated from PMII by weak acid hydrolysis and separation by SEC and HPAEC-PAD. The isolated oligosaccharides were desalted, reduced and methylated. GC-MS analysis of the partially methylated alditol acetates has been used to reveal the structure of the oligosaccharides. 

PA-1 was partially hydrolyzed with O.IM TFA (40°C, 24 h) and the three fractions (PA-l-I, PA-l-II and PA-l-III) were obtained by Bio-gel P-6. Especially, about 50% of TBA-positive material (PA-l-III) was eluted in the fraction of small oligosaccharide. Permethylated oligosaccharide-alditols from PA-l-III were analyzed by GC-EIMS, and three disaccharide-alditols (IP, 2P and 3P) were detected. EI-MS and component sugar analysis suggested that the major peak, IP was Rha-(l- 5)-Kdo-ol and the minor peaks, 2P and 3P were two epimers of Araf-( l->5)-Dha. 

Methylation analysis Permethylation of intact oligosaccharides Hydrolysis of glycosidic linkages Reduction to monosaccharide alditols Acetylation of free hydroxyl groups previously involved in link-... 

This oxidation has been used to determine the anomeric nature of sugar residues in oligosaccharides.153 The oligosaccharide is reduced to the alditol, this is acetylated, and the ester is treated with chromium trioxide in acetic acid in the presence of an internal standard. From the sugar analysis of the product, the residues that have survived (and, consequendy, are a-D-linked) may be identified. The... 

M. K. Fatema, H. Nonami, D. R. B. Ducatti, A. G. Gonqalves, M. E. R. Duarte, M. D. Noseda, A. S. Cerezo, R. Erra-Balsells, and M. C. Matulewicz, Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analysis of oligosaccharides and oligosaccharide alditols obtained by hydrolysis of agaroses and carrageenans, two important types of red seaweed polysaccharides, Carbohydr. Res., 345 (2010) 275-283. 

W. Morelle, J. Lemoine, G. Strecker, Structural analysis of 0-linked oligosaccharide-alditols by ESI-MS-MS after mild periodate oxidation and derivatization with 2-PA, Anal. Biochem., 259 (1998) 16. 

The He of the freshwater snail L. stagnalis has a molecular mass of about 9x10 kDa and a carbohydrate content of 3% (Table 3). So far, no detailed amino acid sequence data are available. Analysis of the glycosylation pattern of the He (hydrazinolysis procedure oligosaccharide-alditols) afforded a series of 8 oligosaccharide structures (Scheme 4) [22,23]. The low-molecular-mass oligosaccharide is a xylosylated core... 

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