Alcohol dehydrogenase molecular weight

Horse liver alcohol dehydrogenase has a molecular weight of 80 000 and is made up of two subunits, each containing two zinc atoms. The subunits are not active in the monomeric form. 

Alcohol dehydrogenase is also obtained from yeast. Yeast alcohol dehydrogenase (YADH) was the first pyridine nucleotide-dependent dehydrogenase to be crystallized by Negelein and Wulff in 1937.1342 YADH is a tetramer of molecular weight 140000-150000. The amount of zinc determined varies,1343 but there are strong indications that the subunits of YADH and LADH have similar structures, including the presence of two zinc atoms. 

Leca and Marty [26] Cider Wine Whisky Co-immobilisation of alcohol dehydrogenase (ADH), NADH oxidase and high molecular weight NAD (NAD-dextran) in a poly(vinyl alcohol) bearing stryrylpyridinium groups (PVA-SbQ) matrix Pt/+550 vs. Pt ... 

Ketone and Aldehyde Reduction. In addition to the reduction of aldehyde and ketones through the reverse reaction of alcohol dehydrogenase, a family of aldehyde reductases also reduces these compounds. These reductases are NADPH-dependent, cytoplasmic enzymes of low molecular weight and have been found in liver, brain, kidney, and other tissues. 

Schulze et al. [135] developed fused-silica chips dynamically coated with hydroxypropylmethyl cellulose and utilized them for the separation of aromatic low molecular weight compounds such as serotonin, propranolol, a diol, and tryptophan. The authors used deep UV laser-induced fluorescence detection for these compounds. Schuchert-Shi et al. [136] identified ethanol, glucose, ethyl acetate, and ethyl butyrate, byproducts obtained in enzymatic conversions using hexokinase, glucose oxidase, alcohol dehydrogenase, and esterase. The authors reported that the quantification for ethyl acetate was possible using contactless conductivity detection. Hu et al. [137] described the separation of reaction products of (3-thalassemia in a multiplex primer-extension reaction using NCE. The method developed was used for patient samples and the results coincided with those of a detection kit. 

TABLE 4-6. Molecular Weight and Polydispersity of MWL Preparations and Dehydrogenase Polymers (DHP) of Coniferyl Alcohol"... 

Figure 2. SDS gel electrophoresis of the products of partial cystine cleavage for several test proteins. A. molecular weight standards, B. yeast alcohol dehydrogenase. C. P-lactoglobulin, D. hen egg lysozyme, E. ovalbumin, F. calf fetal serum fetuin. Molecular weight standards are indicated by arrows on the left side of the gel and are bovine serum albumin (66,300), bovine liver glutamate dehydrogenase (55,400), porcine muscle lactate ddiydiogenase (36,500), bovine erythrocyte carbonic anhydrase (31,000), soybean trypsin inhibitor (21,500), hen egg lysozyme (14,400), bovine lung aprotinin (6,000), unresolved bovine pancreatic insulin A and B chains.

A. Lane 1, Molecular weight markers lane 2, Purified final product. Molecular mass markers are Novex SeeBlue Pre-Stained Standards and range as follows (from top to bottom) Myosin, 250-kDa BSA, 98-kDa Glutamic dehydrogenase, 64-kDa Alcohol dehydrogenase, 50-kDa Carbonic anhydrase, 36-kDa Myoglobin, 30-kDa Lysozyme, 16-kDa Aprotinin, 6-kDa Insulin B chain, 4 kDa. 

For example, human alcohol dehydrogenase exists in more than twenty forms.6 These isoenzymes are all dimers, and all have molecular weights of 80 kDa, or 40 kDa per subunit. The different subunits have been called the a, p, yl, y2, n, and X subunits. All of the isoenzymes catalyze the conversion of ethanol to acetaldehyde according to Eq. 10.1 ... 

Another important, low molecular weight flavor compound is acetaldehyde. This material plays a significant role in the flavor of yogurt and certain fruit flavors, such as orange. A procedure was patented in 1984 for the conversion of ethanol to acetaldehyde (17) using the enzyme alcohol dehydrogenase (ADH). 

Abbreviations are HC, hemocyanin from Helix pomatiar IG, nonspecific human y-im-munogiobulin AD, alcohol dehydrogenase from yeast Hb, human adult carbonmonoxy-hemoglobin CA, human erythrocyte carbonic anhydrase B and LY, hen egg-white lysozyme. The numbers next to the abbreviations give the molecular weights in units of thousands of Daltons. The relaxation rate of the protein-free solvent is indicated by the horizontal dashed line. After Ref. 7. 

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