Analytical methods high-throughput

The application of combinatorial principles in chemical synthesis, particularly in the search for active substances, requires analytical methods with high throughput (von dem Bussche-Hunnefeld et al. [1997]). Screening techniques can be used to analyse a large number of test samples in a short... 

The relative simplicity of the sensor setup allows them to be implemented into portable automated devices or bed-side analyzers (Fig. 4.2), which are easily installed at patient beds, eliminating the time-consuming laboratory analyses. On the other hand, modem high throughput clinical analyzers may process more than 1000 samples per hour and simultaneously determine dozens of analytes, using a handful of analytical methods. Blood electrolyte analysis, however, remains one of the most important in... 

Recent articles about new sorbents and applications of high-throughput sample preparation methods in conjunction with HPLC/MS/MS analysis of analytes from biological matrices are included at the end of the reference list below. Spatial considerations prevented detailed discussions in this chapter. The articles appear as References 197 through 212 on the list below. 

TDM has improved the performance of anticancer, antidementia, antidepressant, antiepileptic, anticonvulsant, antifungal, antimicrobial, antipsychotic, antiretroviral, anxiolytic, hypnotic, cardiac, addiction treatment, immunosuppressant, and mood stabilizer drags for more than 30 years.2-9 Many analytical procedures evolved as analytical techniques and instrumentation have advanced. This chapter briefly reviews the different types of analytical methods the applications of high-throughput techniques in TDM are discussed in detail. 

Salm et al.44 developed a high-throughput analytical method to measure cyclosporine in whole blood. They used a simple SPE procedure, followed by HPLC-MS/MS. An Agilent 1100 liquid chromatograph was coupled with an Agilent Zorbax Bonus C18 reversed-phase column (50 x 2.1 mm, 5 jt/rn particle size). The column temperature was maintained at 70°C in a column oven. The mobile phase consisted of 80% methanol and 20% 40mM ammonium acetate buffer (pH 5.1) delivered isocratically at a flow of 0.4 mL/min. D12 cyclosporine was the IS. 

Dr. Perry G. Wang is currently a principal scientist at Teleflex Medical. His interests include analytical method development and validation, medicated device products, and environmental engineering. His expertise focuses on high-throughput analysis of drugs and their metabolites in biological matrices with LC/MS/MS. 

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