Electrophoresis agarose

The use of agarose as an electrophoretic method is widespread (32—35). An example of its use is in the evaluation and typing of DNA both in forensics (see Forensic chemistry) and to study heritable diseases (36). Agarose electrophoresis is combined with other analytical tools such as Southern blotting, polymerase chain reaction, and fluorescence. The advantages of agarose electrophoresis are that it requires no additives or cross-linkers for polymerization, it is not hazardous, low concentration gels are relatively sturdy, it is inexpensive, and it can be combined with many other analytical methods. 

Serum is separated by agarose gel electrophoresis and the gel is stained for lipids [45, 74]. Agarose electrophoresis separates four major lipoproteins (i.e., chylomicrons,... 

Restriction patterns produced by agarose electrophoresis of DNA fragments after restriction endonuclease action. Courtesy of Bio-Rad Laboratories, Richmond, CA. 

Extrachromosomal DNA molecules called plasmids are harbored in some strains of E. coli. The normal copy number of the plasmids is small, between 2 and 10 however, if these strains of E. coli are grown in the presence of chloramphenicol, up to 3000 copies may be replicated per cell. Plasmid DNA has been demonstrated to be a useful vehicle in molecular cloning. This experiment describes a method for the growth of E. coli and amplification of the ColEl plasmids. The plasmids will be isolated from E. coli cells by one of two methods, a large-scale boiling method or a microscale alkaline lysis method. The DNA plasmids will be measured for molecular size by agarose electrophoresis. 

Two isolation procedures based on methods in category 2 are described in this experiment, a large-scale and a microscale method. Each procedure yields plasmid DNA that is sufficiently pure for size analysis by agarose electrophoresis and for digestion by restriction enzymes as described in Experiment 15. 

Resuspend the pellet of DNA in 25 /xL Tris-EDTA, pH 8.0 buffer. Save the plasmid prep for agarose electrophoresis (part C) and use in Experiment 15. 

Assume the reaction digest from Problem 5 is analyzed by agarose electrophoresis. Draw an electrophoresis gel and show the approximate location of each DNA fragment. 

The use of agarose as an electrophoretic method is widespread. The advantages uf agarose electrophoresis are that it requires no additives of cross-linkers for polymerization, it is not hazardous, low concentration gels are relatively sturdy, it is inexpensive, and it can be combined with many other analytical methods. 

Soto CM, Blum AS, Wilson CD, Lazordk J, Kim M, Gnade B, Ratna BR (2004) Separation and recovery of intact gold-virus complex by agarose electrophoresis and electroelution application to the purification of cowpea mosaic virus and colloidal gold complex. Electrophoresis 25 2901-2906... 

X Taq Polymerase buffer (supplied with enzyme)— Gibco BRL PCR thermocycler Agarose (electrophoresis grade)... 

Agarose electrophoresis utilizes agarose gels that have large pores and are used to separate DNA and RNA (200-50000 base pairs). 

Figure 4-29. Determination of the size of double stranded DNA using agarose gel electrophoresis. The pattern of bands from DNA standards of varying lengths following agarose electrophoresis is shown on the left. A semi-...

Mutation Screening by Ultrathin-Layer Agarose Electrophoresis... 

A characteristic feature of apoptosis is DNA fragmentation. Wyllie (22) described, in association with apoptosis, nucleosomal fragmentation which can be seen by agarose electrophoresis with a ladder of DNA bands representing... 

Most routine procedures for agarose electrophoresis today are carried out on commercially produced, prepackaged microzone gels, and the sample is applied by means of a thin plastic template with small slots corresponding to sample application points. The template is placed on the agarose surface, and 5- to 7- LtL samples are placed on each slot. The serum sample is allowed to diffuse into the agarose for 5 minutes, excess sample is removed by blotting, and the... 

Katzmann JA, Clark R, Namyst-Goldberg C, Sanders L, Kyle RA, Landers JP. Identification of monoclonal proteins by capillary electrophoresis quantitative comparison with acetate and agarose electrophoresis. Electrophoresis 1997 18 1775-80. 

Electrophoresis at pH 6.4 using a citrate buffer is performed when an abnormal band is noted on alkaline Hb electrophoresis. Agarose is the preferred medium, with Acid Violet the preferred stain. Figure 31-10 shows the same Hb variants performed on agarose electrophoresis at pH 6.4 and stained with Acid Violet. The order of migration (cathode to anode, fastest to slowest) is Hb F, Hb A, Hb S, and Hb C. Hemoglobins D, G, I, J, O, A2, and E co-migrate with Hb A. 

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