Cowpea mosaic virus

Biopharmaceuticals in Plants Toward the Next Century of Medicine 

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Hibi, T., Rezelman, G. and Van Kammen, A. (1975). Infection of cowpea mesophyll protoplasts with cowpea mosaic virus. Virology 64, 308-18. 

Rezelman, G., van Kammen, A. and Wellink, J. (1989). Expression of cowpea mosaic virus mRNA in cowpea protoplasts. J. Gen. Virol. 70, 3043-50. 

Cowpea mosaic virus Fusion with CP, fusion to CP with protease cleavage site, complementation GFP, HIV, MEV, HRV, MV, CPV, S. aureus, Ps. aeruginosa 11, 12, 13, 31, 43, 58... 

Canine parvovirus (CPV) VP2 epitope Epitope display on cowpea mosaic virus in cowpea leaf Immunogenic in mice when delivered parenter-ally or nasally. Dogs developed 3L17- and VP2-specific IgG sera neutralized CPV in vitro. Protective against lethal challenge in dogs immunized parenterally. 76, 77... 

Foot and mouth disease virus VP1 epitope Cowpea mosaic virus in cowpea leaf No immunogenicity assays performed. - 79... 

Human rhinovirus type 14 VP1 epitope Epitope display on cowpea mosaic virus in cowpea leaf Immunogenic in rabbits when delivered parenterally. 81... 

Staphylococcus aureus D2 epitope of fibro-nectin-binding protein (FnBP) Cowpea mosaic virus in cowpea leaf Potato vims X in tobacco leaf Elicited FnBP-specific IgA and IgG. Immunogenic in mice and rats when delivered orally, nasally, or parenterally. 85... 

Charterji, A., Bnms, L.L., Taylor, S.S. et al. (2002). Cowpea mosaic virus from the presentation of antigenic peptides to the display of active biomaterials. Intervirology 45 362-370. 

Gopinanth, K., Wellink, J., Porta, C. et al. (2000). Engineering cowpea mosaic virus RNA-2 into a vector to express heterologous proteins in plants. Virology 267 159-173. 

Porta, C., Spall, V.E., Eindlay, K.C., Gergerich, R.C., Earrance, C.E., and Lomonossoff, G.R (2003). Cowpea mosaic virus-based chimeras effects of inserted peptides on the phenotype, host-range and transmissibility of the modified viruses. Virology 310 50-63. 

Porta, C., Spall, V.E., Loveland, 1., lohnson, I.E., Barker, P.J., and Lomonossoff, G.P. (1994). Development of cowpea mosaic virus as a high-yielding system for the presentation of foreign peptides. Virology 202 949-955. 

Verver, J., Wellink, J., and Van Lent, J. et al. (1998). Studies on the movement of cowpea mosaic virus using the jellyfish green fluorescent protection. Virology 242 22-27. 

Fig. 7.9. Backscattered ICP Raman (/R + /L) and ROA (/R — /L) spectra measured for (a) the empty cowpea mosaic virus (CPMV) protein capsid (top pair), (b) the intact capsid containing RNA-2 (middle pair), and (c) the difference spectra obtained by subtracting the top from the middle spectra to reveal the spectra of the viral RNA-2 (bottom pair)...

Soto CM, Blum AS, Wilson CD, Lazordk J, Kim M, Gnade B, Ratna BR (2004) Separation and recovery of intact gold-virus complex by agarose electrophoresis and electroelution application to the purification of cowpea mosaic virus and colloidal gold complex. Electrophoresis 25 2901-2906... 

Plant viruses Cowpea mosaic virus Icosahedron/28nm diameter The reader is referred to references throughout the text and Table 9.2... 

Figure 9.1 Trifoliate leaves from Vigna unguiculata. Left infected with Cowpea mosaic virus. Right noninfected leaf.

Figure 9.2 Transmission electron micrograph of Cowpea mosaic virus particles negatively stained with uranyl acetate. The scale bar is lOOnm.

Figure 9.3 The structure of the Cowpea mosaic virus (CPMV) capsid (a) and the asymmetric unit (b). The capsid of CPMV is comprised by the small (S) and large (L) subunit.

Figure 9.4 The asymmetric unit of the capsid of the Cowpea mosaic virus. Depicted are addressable surface residues (highlighted as spheres), (a) Addressable lysines lys 38 and lys 82 on the small (S) subunit (in dark gray), lys 34, lys 99, and lys 199 on the large...

Table 9.2 Overview of chemical moieties that have been attached to wild-type (wt) and mutant Cowpea mosaic virus (CPMV) particles and the purpose of the study and/or application.

Figure 9.6 (a) Schematic presentation of Cowpea mosaic virus (CPMV) particles chemically modified with ferrocenecarboxylic acid (Fc) moieties via the formation of a peptide bond, (b) Cyclic voltammogram of derivatized CPMV-Fc at a scan rate v ofO.lVs-1, and linear plot of current versus (scan rate)l/2 (inset). 

Figure 9.8 Bilayers and a mixed monolayer of biotinylated and fluorescently labeled Cowpea mosaic virus (CPMV) particles on gold slides imaged via fluorescence microscopy The bilayers are shown on the left and the mixed monolayer on the right. A schematic representation of the CPMV layer...

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