Agarose and Paper Electrophoresis

Identify the protein spots as usual, i.e., by staining, autoradiography, gel overlay, or Western blot. In the latter case the gel must be separated from the GelBond foil prior to electrotransfer. For this purpose a film remover (Gorg 2003, Fig. 19) is used The gel is placed on the cylindrical remover with foil down, clamped on an edge, and a thin stainless steel or nylon wire is pulled between foil and gel towards to your body. Cover the gel with the wetted blotting membrane (cf Protocol 2.4.3) and transfer membrane as well as gel to the blotting apparatus. 

O Farrel PH (1975) J Biol Chem 250 4007 Dunn MJ, Burghes AHM (1983) Electrophoresis 4 97 4 173 Gorg A, Postel W, Gunther S (1988) Electrophoresis 9 531 Righetti PG (1990) Immobilized pH gradients theory and methodology. Laboratory techniques in biochemistry and molecular biology, vol. 20, Elsevier, Amsterdam 

Westermeier R (1997) Electrophoresis in practice a guide to methods and applications, 2nd ed. VCH, Weinheim GOrg A, Obermaier C, Boguth G, Harder A, Scheibe B, Wildgruber R, Weiss W (2000) Electrophoresis 21 1037 

GOrgA(2003) Two-dimensional electrophoresis with immobilized pH gradients for proteome analysis, http //www.weihenstephan.de/blm/deg 

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