Agar identification

Borjesson T., Stdllman U. and Schniirer J.L. (1993) Compounds produced by molds on oatmeal agar. Identification and relation to other growth eharacteristics. J. Agric. Food Chem., 41, 2104-2111. 

Concentration by membrane filtration. Inoculation on a pre-enriched medium. Enrichment, subculturing on isolating agar. Identification. 

Fukasawa, S., Dunlap, P. V., Baba, M., and Osumi, M. (1987). Identification of an agar-digesting, luminous bacterium. Agric. Biol. Chem. 51 265-268. 

Bismuth sulphite agar. This medium was developed in the 1920s for the identification of Salmonella typhi in water, faeces, urine, foods and pharmaceutical products. It consists of a buffered nutrient agar containing bismuth sulphite, ferrous sulphate and brilliant green. 

The colony characteristics and microscopic morphology used for the identification of dematiaceous fungi are based upon cultures that are approximately 2 weeks old and have been grown at 25 to 30°C on a medium such as potato dextrose agar or cornmeal agar. These media... 

AHLs can be tentatively identified by comparison of the unknown with synthetic AHL standards after Thin Layer Chromatography (TLC) in which the plates are overlaid with agar containing one of the AHL biosensors described above [37,39,44,45]. However, for the unequivocal identification of AHLs the use of more powerful methods such as LC-mass spectrometry, nuclear magnetic resonance and infrared spectroscopy as described below are required. 

Agar JN, Yuvaniyama P, Jack RF, et al. 2000b. Modular organization and identification of a mononnclear iron-binding site within the NifU protein. J Biol Inorg Chem 5 167-77. 

High voltage electrophoresis (HVE) in agar gel with detection by bioautography has been used with considerable success in some laboratories for identification of residues (1-6). This procedure has the advantage that all antibiotic substances detectable by bioautography can be classified on the basis of electrophoretic mobility. Further testing may be required for quantification and to... 

An effective approach of antimicrobial therapy of an infection is based on the isolation and identification of the infected organism and determining its sensitivity to antimicrobial drngs. In vitro tests, snch as diffnsion in agar and determining the minimally inhibitory concentration in a liqnid medinm are the most widely used tests. 

Figure 5.10 Agar overlay screening procedure to screen microbial populations for hydantoinase activity (Morin, Hummel and Kula, 1986). A screen for dihydropyiidinase activity based on Schiff base formation with PDMB (upper panel) led to the identification of numerous strains with D-hydantoinase activity, which in combination with a D-carbamoylase is employed to produce D-amino acids.

Pseudomonas sample of 100 ml shall be filtrated and placed the membrane filter into 50 ml of TSB incubated for 20 to 28 hours at 35 2°C. Transfer into Pseudomonas agar F and P for 40 to 48 hours at 35 2°C. If any characteristic colony is detected, identify using API and/or automated identification system... 

Coliform using membrane hltration techniques, hltrate 100 ml. Place the membrane filter into Endo agar and identify using API and/or automated identification system. 

A sensitive and specific bioautographic method for the identification of choline and its derivatives, was developed by Lewin and Marcus, utilizing a Neurospora crassa mutant [5]. As little as 0.03 y of choline chloride is detectable. The applicability of the method for detection of choline derivatives in lipid hydrolyzate is discussed. A method for determining the rate of diffusion of compounds from paper chromatograms into bioautograph agar is described. 

To detect low levels of bacteria that might be missed with the quantitative technique, 5 pL of a biological fluid is inoculated into 5 mL of trypticase-soy broth and incubated overnight at 37°C. If bacterial growth is identified, the colonies are seeded onto a tryptic soy blood-MacConkey agar plate for further identification. 

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