Aerosolized vector

Direct application of the tgAAVCF vector to the nasal epithelium and a single lung lobe revealed no troubling safety issues, but it also did not provide evidence of efficient gene transfer or expression at the sites of administration. However, evaluation of aerosolized vector delivery to CF patients provided data that indicated that AAV vectors could produce gene transfer in the lung. 

Secondary Hazards Aerosols (possibly) Fecal matter Vectors (mechanical). 

Secondary Hazards Aerosols (body fluids) Blood and body fluids Body tissue Vectors (mechanical—rodents). 

Secondary Hazards Aerosols (cough, sneeze, contaminated dust) Contact Body fluids Fecal matter Fomites Vectors (mechanical—stable fly). 

Secondary Hazards Aerosols (blood, fluids, contaminated dust) Fomites (with vectors). 

Secondary Hazards Aerosols (cough, sneeze) Contact Vector cycle. 

Secondary Hazards None Mechanical vectors (planting, harvesting, track out) Aerosols Spores Crop debris. 

A second approach involves direct injection/administration of the nucleic-acid-containing vector to the target cell, in situ in the body. Examples of this approach have included the direct injection of vectors into a tumour mass, as well as aerosol administration of vectors (e.g. containing the cystic fibrosis gene) to respiratory tract epithelial cells. 

Probable Form of Dissemination Aerosol infected vectors (carrier organism). Detection in the Field None. 

Pneumonic) Plague 1. Aerosol 2. Infected vectors High High 2-3 days 1-2 days Very high Less important because of high transmissibility Yes Moderately effective No... 

Rocky Mountain Spotted Fever 1. Aerosol 2. Infected vectors No High 3-10 days 2 weeks to months High Not very stable No Effective No... 

Several vectors have been used in an attempt to deliver the cf gene to the airway epithelial cells of sufferers. The most notable systems include adenoviruses and cationic liposomes. Vector delivery to the target cells can be achieved directly by aerosol technology. Delivery of cftr cDNA to airway epithelial cells (and subsequent gene expression) has been demonstrated with the use of both vector types. However, in order to be of therapeutic benefit, it is essential that 5-10% of the target cell population receive and express the cftr gene. This level of integration has not been... 

Answer Aerosol delivery of the CFTR gene. Both viruses and liposome-DNA complexes are capable of successful CFTR gene transfer to the nasal and airway epithelia of patients with CF. In fact, gene transfer to the airways is one of the few areas where liposome-DNA complexes match the expression obtained using viral vectors without the viruses inflammatory side effects. Current trials are aimed at optimizing gene delivery with reduced toxicity to produce sustained correction of the epithelial transport defect. 

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