Hemoglobin adsorption

A fourth separation technique employs charcoal. Charcoal has adsorption characteristics such that most organic molecules will adhere to its surface. However, charcoal is also very porous, thereby allowing smaller molecules to enter into its pores and become trapped in them. The charcoal is first coated with dextran or hemoglobin which covers its outer surface. 

LiNa Geng et al. realized similar experiments for the adsorption of trypsin [141] and hemoglobin [142] on y-zirconium phosphate (y-ZrP) and organo y-ZrP intercalated with butylammonium (BA) and tetrabutylammonium (TBA). Hb adsorbed in the galleries of BA-y-ZrP and TBA-y-ZrP mainly by hydrophobic... 

Isotherm Subtraction. A second method (7) of determining the net proton coefficient from adsorption data is an adaptation of the thermodynamics of linked functions as applied to the binding of gases to hemoglobin (19). The net proton coefficient determined by this method is designated, Xp- The computational procedure makes a clear distinction between the influence of adsorption density and pH on the magnitude of the net proton coefficient. The fundamental equation used in the calculation of Xp is... 

Bimer, G Neumatm, H.-G (1988) Biomonitoring of aromatic amines. II Hemoglobin binding of some monocyclic aromatic amines. Arch. Toxicol., 62, 110-115 Bollag, J.-M., Blattmann, P. Laanio, T. (1978) Adsorption and transformation of four substituted anilines in soil. J. Am. Food Chem., 26, 1302-1306 Bull, D.L. (1973) Metabolism of chlordimefon in cotton plants. Environ. EntomoL, 2, 869-871 Chemical Information Services (1999) Directory of World Chemical Producers (Version 99.1.0) [CD-ROM], Dallas, TX... 

FIGURE 6.2 Adsorption of hemoglobin on ethylene glycol-coated silica. 

Hemoglobin, Solid, Adsorption of Water on (Eley Leslie). .. 7 238... 

PREFERENTIAL. Descriptive of the selectivity of action, either chemical or physiochemical. exhibited by a substance when in contact with two other substances it may be due either to chemical affinity or to surface phenomena. An example of a preferential chemical combination is that of hemoglobin with carbon monoxide, with which it unites 200 times as readily as it does with oxygen when expose to a mixture of the two. Such phenomena as adsorption, corrosion, and the wetting of dry powders by liquids are other examples,... 

Depending on the fit, the number and strength of contacts, and other factors, a monomer-oligomer equilibrium will exist. For example, in the case of Concanavalin A the tetramer is in equilibrium with dimer and monomer under normal conditions. In the case of insulin the monomer, dimer, and hexamer are all in equilibrium. In hemoglobin the dimer and tetramer are in equilibrium. In the case of the adsorption of such proteins, one must not only know what is the aggregation state of the protein in solution, but must be able to deduce the adsorbed state. Do the molecules adsorb as dimers 21) Do they adsorb as dimers and then associate as tetramers or hexamers on the surface, etc. ... 

Recently Horbettl02) and Brash and ten Hove 103) have quantitatively demonstrated the Vroman effect in a series of experiments studying competitive adsorption of fibrinogen, albumin, IgG, and hemoglobin from diluted plasma. 

As a hemoglobin model, using imidazole attached polystyrene) with the picket fence porphyrin-Co(H) (XIV) the reversible oxygen adsorption-desorption reaction is observed (148,149). 

FIGURE 9 Shows the dynamic capacity curves for hemoglobin adsorption on two membrane module setups (A) two 8 m2 modules in parallel show capacity of 100 g and (B) addition of 4 m2 and I m2 modules in series (as shown in Fig. 8) increased the dynamic capacity to 140 g of hemoglobin. 

The sorption efficiency of MC was determined as the ratio of the quantity of the adsorbed substance to its initial amount (w / w), expressed in % for a certain ratio (w / w) of adsorbent to substance. Optimal ratios of adsorbent to substance equal 15-20 for barbiturates, 20 - 25 for cyanocobalamin and bilirubin, and 40 - 50 for hemoglobin. The initial concentration of absorbed substances was 100 - 200 pg/ml. The substances were incubated for lmin with MC either in physiological solution or in donor plasma and donor blood at room temperature (pH 7.4). The concentration of substances in the solutions was measured by differential visual and UV-spectroscopy. Concentrations of substances in blood and plasma and adsorption of total plasma proteins was determined by thin-layer chromatography with a fluorescent label. 

Heme (7 ) with poly(L-lysine) is slowly oxidized at pH 12 to the oxygenated complex sigmoid curve of O2 adsorption like In hemoglobin also binds CO and CN"... 

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