Activation of Polysaccharides for Covalently Attaching Ligands and Proteins

Various colors of chlorotriazine dyes are available, including Cibacron Blue, Pro-cion Brilliant Blue, Procion Brilliant Red, Procion Brown, Procion Green, and Procion Yellow. 

The dyeing of polysaccharides has been used for three purposes (1) the formation of dyefast fabrics, (2) the visible identification of polysaccharides for study in electrophoresis and gel chromatography [32], and (c) to provide substrates for identification of enzymes in enzyme screening tests and in assaying of specific enzymes such as a-amylase [33], cellulase [34], chitinase [35], dextranases [36], and so forth. 

8 Activation of Polysaccharides for Covalently Attaching Ligands and Proteins 

Polysaccharides and cross-linked polysaccharides can be activated by reaction with alkaline cyanogen bromide (reaction 7.19). Polysaccharides such as cross-linked dextran, agaran, and cellulose have been activated by this reaction [37,38]. The activated polysaccharides can then react with amino groups of various ligands that can be used in affinity chromatography [39], or they can react with amino groups on the surface of enzymes to give immobilized enzymes [40] (reaction 7.20). 

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