Acetate Agar

CK isoenzymes have been separated by electrophoresis on cellulose acetate, agar gel, agarose and polyacrylamide gel. 

A poly (vinylchloride) membrane electrode was described for local anesthetics, based on dibenzo-24-crown-8 as the electroactive material, and di(2-ethyl)hexyl phthalate as the plasticizer [74]. It was reported that the electrode exhibited a Nemstian response to procaine, and other electrode properties were also presented. The analysis was performed at pH 6 to 6.5 vs. S.C.E., with a 0.2 M lithium acetate agar bridge. Less efficient crown ethers studied at this time were benzo-15-crown-5, dibenzo-18-crown-6, and dibenzo-30-crown-10. 

Electrophoretic methods using supporting media, such as starch, starch-gel, cellulose acetate, agar gel, continue to be of importance in detecting abnormal hemoglobin variants. The techniques are less suit-... 

Ascospore formation on acetate agar according to a defined procedure (TF positive)... 

A. Acetate Agar Ascospore formation in Saccharomyces is generally demonstrated using an acetic acid-supplemented agar. As sporulation occurs only under oxidative conditions, glucose concentrations must be kept below 1.0 g/L. The following media is that of McClary et al. (1959) as described in Lodder (1970) ... 

B. Gorodkowa Agar Gorodkowa agar is one of the media used to demonstrate ascospore formation in Pichia sp. Others include YM as well as acetate agar. The following medium is described by Lodder and Kreger-van Rij (1952) as described in Lodder, 1970 ... 

Acetate Agar 2, Gorodkowa, and the V-8 media are described by Yarrow (1998), and the enriched yeast extract-malt extract medium was originally described by van der Walt and van Kerken (1961). The staining procedure for spore cultures using malachite green is from Yarrow (1998). 

Principal characteristics C. intermedius cultures fail to produce HgS in TSI agar, but hydrogen sulfide production is detectable on more sensitive media such as FeClg gelatin or Pb acetate agar lysine is never decarboxylated. About 20 per cent of strains produce indole and 40 per cent ferment sodium malonate [21, 88]. 

Methylprednisone 21-acetate (0.5 g), when hydrolyzed by means of aqueous alcoholic potassium bicarbonate yields 16 fnethylprednisone. An alternative method of the preparation of the compound of this example is as follows. Bacillus sphaericus var. fusifermis (A.T.C.C. 7055) is incubated on a nutrient agar (composed of Bacto-beef extract, 3 g Bacto-peptone,... 

Extraction of Sodium Channel Blockers. A review of published reports shows that methods for purification of sodium channel blockers from bacterial cultures are similar to techniques for isolation of TTX and STX from pufferfish and dinoflagellates (30, 31, 38, 39). Typically, cell pellets of bacterial cultures are extracted with hot 0.1% acetic acid, the resulting supernatant ultra-filtered, lyo-philized, and reconstituted in a minimal volume of 0.1% acetic acid. Culture media can also be extracted for TTX by a similar procedure (Ji). Both cell and supernatant extracts are analyzed further by gel filtration chromatography and other biological, chemical, and immunological methods. Few reports describe purification schemes that include extraction of control samples of bacteriological media (e.g., broths and agars) which may be derived from marine plant and animal tissues. 

The defnon6ttLOtion 0 -chain vaAijant6 In heterozygotes Is complicated by the presence of the large amount of Hb-F. Another obstacle Is the nearly Identical electrophoretic mobilities of Hb-A and the minor Hb-Fi component. Despite these difficulties, abnormalities such as AS, SS, AC, CC, SC, and others can readily be detected using cellulose acetate electrophoresis, starch gel electrophoresis, acid agar electrophoresis, and by CM-Cellulose microchromatography to be described In a separate section. 

Agar may be acetylated with pyridine and acetic anhydride and the resultant acetyl derivative readily converted into the corresponding methyl ether by reaction with sodium hydroxide and methyl sulfate ... 

Mixed Cytokinins. The first cytokinin, kinetin [525-79-1] (3), was isolated from stale herring sperm (8) but, like so many biologically active natural products, it was later found in the vascular system of tobacco stems and leaves (9). Yeast also proved to have a very high titre of kinetin (see Yeasts) (8). The compound is very active in increasing cell division in tobacco wound callus tissue that has been cultured on White s agar medium supplemented with 2 mg/L of indole-3-acetic acid (IAA) [87-51 -4], The presence of IAA is mandatory to induce cell division in the presence of kinetin. 

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