Malt Extract agar

C. cassiicola DSM 62475 was taken from stock culture and incubated for 15 days at 25 °C on malt extract agar plate (consisting of 30 g malt extract, 3 g peptone and 17 g agar in 1 L distilled water adjusted to pH 5.6 with AcOH and sterilized at 121 °C for 20 min). 

Claviceps will grow on various organic substrates. Potato Dextrose Agar (PDA) and Malt Extract Agar (MEA) are the most popular. 

Perhaps ASTM E1428 (a. 16), commonly referred to as the Pink Stain test, is one of the most recognised plastic test methods. The actinomycete Streptoverticillium reticulum is used as an indicator organism to inoculate yeast malt extract agar. Test discs of plastic are placed on the surface and incubated at 29 °C for three weeks. Assessment focuses on the extent of staining of the... 

MEA Malt Extract Agar) 20 grams tan malt 2 grams yeast... 

Brucella agar(Difco) for bacteria, Yeadt extract-malt extract agar(lSP medium No. 2) for actinomycetes, SG(Soybean-glycerol) agar for Streptomyces flaveus Y-1. 

Malt Extract Agar Fungi, e.g., Podosphaera castagnei, mold, mildew 48h, 25°C... 

Secondary testing (either traditional plating or rapid methods) tends to only be used if ATP levels are consistently breaching tolerance limits and/or are not reduced by additional cleaning. Malt extract agar or similar may be used for the detection of acetic and lactic bacteria and wild yeast. 

C. Malt Extract Agar Malt extract agar is used to sporulate Schizosaccha-romyces and Hansenula sp. and is marketed by several supply houses. Prepare according to manufacturer s instructions. Where malt extract (but not malt extract agar) is available, the broth may be solidified by inclusion of 2% (wt/vol) agar prior to autoclaving. Upon completion of the autoclave cycle, cool and pour plates as previously described. 

E. V-8 Agar V-8, along with malt extract agar, is used to sporulate Hansenula sp. The following medium is that of Wickerham et al. (1946) as described in Lodder (1970) ... 

Agar (granulated) Malt agar Malt extract agar Peptone... 

Enriched Yeast Extract-Malt Extract Agar... 

Sample collections were performed in March of 2014. Air samples of 50 L were collected through an impaction method with a flow rate of 140 L/ min onto Malt Extract Agar (MEA) supplemented with chloramphenicol (0.05%), using the Millipore air Tester (Millipore). Surfaces samples were collected by swabbing the surfaces of the same indoor sites, using a 10 by 10 cm square stencil disinfected with 70°/o alcohol solution between samples according to the International Standard ISO 18593 (2004). The obtained swabs were then plated onto MEA. 

The fungus was obtained from the American type culture collection (ATCC) as a freeze-dried ampoule containing dried cell material. The culture was revived by suspension of this material, under aseptic conditions, in sterile water, and its dispersion onto a solid growth medium composed of malt extract agar. The agar... 

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