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Absorption nucleic acid

Nickel 10 mg Interaction with iron absorption, nucleic acid, lipid metabolism Unknown Unknownb... [Pg.762]

One of the most important and exciting advances in modern biochemistry has been the application of spectroscopic methods, which measure the absorption and emission of energy of different frequencies by molecules and atoms. Spectroscopic studies of proteins, nucleic acids, and other biomolecules are providing many new insights into the structure and dynamic processes in these molecules. [Pg.99]

Another property of pyrimidines and purines is their strong absorbance of ultraviolet (UV) light, which is also a consequence of the aromaticity of their heterocyclic ring structures. Figure 11.8 shows characteristic absorption spectra of several of the common bases of nucleic acids—adenine, uracil, cytosine, and guanine—in their nucleotide forms AMP, UMP, CMP, and GMP (see Section 11.4). This property is particularly useful in quantitative and qualitative analysis of nucleotides and nucleic acids. [Pg.330]

The Ultra-violet Absorption of Some Degraded Deoxypentose Nucleic Acids," W. G. Overend, A. R. Peacocke, and M. Stacey, Trans. Faraday Soc., 50 (1954) 305. [Pg.29]

This technique provides an easy and convenient method to evaluate the association of small molecules to various polymorphic forms of nucleic acid structures from the measurement of absorbance changes in the absorption maximum of the hgand, where the nucleic acid has no absorbance. Information about overall DNA/RNA base preference and nature of binding can also... [Pg.167]

Upon absorption of UV radiation from sunlight the bases can proceed through photochemical reactions that can lead to photodamage in the nucleic acids. Photochemical reactions do occur in the bases, with thymidine dimerization being a primary result, but at low rates. The bases are quite stable to photochemical damage, having efficient ways to dissipate the harmful electronic energy, as indicated by their ultrashort excited state lifetimes. It had been known for years that the excited states were short lived, and that fluorescence quantum yields are very low for all bases [4, 81, 82], Femtosecond laser spectroscopy has, in recent years, enabled a much... [Pg.293]

Most HPLC instruments monitor sample elution via ultraviolet (UV) light absorption, so the technique is most useful for molecules that absorb UV. Pure amino acids generally do not absorb UV therefore, they normally must be chemically derivatized (structurally altered) before HPLC analysis is possible. The need to derivatize increases the complexity of the methods. Examples of derivatizing agents include o-phthaldehyde, dansyl chloride, and phenylisothiocyanate. Peptides, proteins, amino acids cleaved from polypeptide chains, nucleotides, and nucleic acid fragments all absorb UV, so derivatization is not required for these molecules. [Pg.479]

UV or visible absorption spectra of proteins and nucleic acids are very simple, with either one or two absorption peaks. Examples are shown in Figure 16.14. [Pg.480]

Ogul chansky TYu, Losytskyy MYu, Kovalska VB, Yashchuk VM, Yarmoluk SM (2001) Interactions of cyanine dyes with nucleic acids. XXIV. Aggregation of monomethine cyanine dyes in presence of DNA and its manifestation in absorption and fluorescence spectra. Spectrochim Acta A Mol Biomol Spectrosc 57 1525-1532... [Pg.155]

The reaction of the p-nitrophenyl esters with the polymer (4) was studied in dimethyl sulfoxide ( DMSO ) solution in the presence of triethylamine at 25°C. The poly-L-lysine derivatives obtained have different IR absorption spectra from those of the starting compounds, and have absorptions assigned to the nucleic acid bases. Poly( e,N-Ade-L-lysine )(5) was soluble in DMSO and ethylene glycol, and also in water below pH 3, where it was present as a protonated form. In dimethylformamide (... [Pg.361]

In relation to these works, the reaction of p-nitrophenyl esters with optically active poly( propyleneimine )(8) was studied at 25°C in DMSO solution according to the same procedure described for the case of poly-L-lysine derivatives. The poly( propyleneimine ) derivatives thus obtained have different IR and UV absorption spectra from those of the starting compounds, and show absorptions assigned to the nucleic acid bases. However, their contents determined by UV spectroscopy were substantially low as compared with the case of poly-L-lysine derivatives for (9) and (20), the base contents were below 30 and 50 %, respectively. The result was explained by a steric hindrance caused by methyl groups on the main chain of poly( propyleneimine ) ... [Pg.361]

The protection of a reactive intermediate complex by the DNA double helix versus a neutral oxidising agent in solution, has also been demonstrated by studying a photo-electron transfer process. In this example the intermediate complex is produced photochemically on the DNA, and is examined spectroscopically after a laser pulsed excitation [73]. Thus Ru(TAP)2(HAT) physically bound to nucleic acid is photo-reduced by hydroquinone during the laser pulse. The intermediate [Ru(TAP)2(HAT)] so-produced, detected by its absorption at 480 nm, is reoxidised by benzoquinone purposely added as oxidant to the solution. It is shown that this reoxidation of the mono-reduced complex is slower in the presence of polynucleotide than in its absence, indicating a protection of the transient mono-reduced complex in the DNA grooves. [Pg.54]

Christensen. E., and A. C. Giese. Changes in absorption spectra of nucleic acids and their derivatives following exposure to ozone and ultraviolet radiations. Arch. Biochem. Biophys. 51 208-216. 1954. [Pg.378]

Optical Spectroscopy General principles and overview, 246, 13 absorption and circular dichroism spectroscopy of nucleic acid duplexes and triplexes, 246, 19 circular dichroism, 246, 34 bioinorganic spectroscopy, 246, 71 magnetic circular dichroism, 246, 110 low-temperature spectroscopy, 246, 131 rapid-scanning ultraviolet/visible spectroscopy applied in stopped-flow studies, 246, 168 transient absorption spectroscopy in the study of processes and dynamics in biology, 246, 201 hole burning spectroscopy and physics of proteins, 246, 226 ultraviolet/visible spectroelectrochemistry of redox proteins, 246, 701 diode array detection in liquid chromatography, 246, 749. [Pg.6]

Several direct methods are available to analyze the tertiary structure of ODNs like nuclear magnetic resonance (NMR) and X-ray crystallographic (XRC) techniques, which needs a sophisticated setup and infrastructure. An alternative but indirect method to study the structure and conformations of nucleic acids is circular dichroism spectroscopy (CD spectroscopy) (25, 26), where circular dichroism refers to the differential absorption of left and right circularly polarized light (27). [Pg.48]

The UV absorption of nucleic acids depends strongly on their nature and solvent conditions as pH, ionic strength, and temperature. Approximate absorption coefficients are given in Tables 1.8 and 1.9. Using these coefficients, yield is calculated during chromatography, ultracentrifugation, and other preparative processes with sufficient precision. [Pg.17]

Table 1.8. Absorption coefficients of nucleotides (nucleic acids)... Table 1.8. Absorption coefficients of nucleotides (nucleic acids)...

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See also in sourсe #XX -- [ Pg.12 , Pg.16 ]




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