A-Methyl-d-mannoside

Methyllepidine, 26, 47 8-Methyllepidine, 26, 47 a-Methyl-d-mannoside, 22, 86 Methyl mercaptan, 26, 9 N-Methyl-(3-methoxy-4-hydroxy-... 

A solution of 200 g. (1.03 moles) of a-methyl-d-mannoside (Org. Syn. Coll. Vol. 1, 1st Ed. (1932), p. 362 2nd Ed. (1941), p. 371) in 3.2 1. of 2 N sulfuric acid (178 cc. concentrated sulfuric acid diluted to 3.2 1.) is steam-distilled from a 5-1. flask for one hour. The flask is heated externally during this time so that the volume of the solution remains constant. The mixture is transferred to a 2- or 3-gal. crock, 10 g. of decolorizing charcoal (Norite) is added, and the solution is rapidly stirred while it is neutralized (litmus) by the addition of barium carbonate (Note 1). 

The plant lectin concanavalin A, a metalloprotein isolated from the jack bean, has also received considerable attention. (588-590,630,631) Using the stopped flow NMR technique three distinct conformation states of the protein, when it is bound to Mn, Ca, and a-methyl-D-mannoside, (630) have been deduced. 

TLC and HPLC. To determine the best solvents for HPLC, 5pl of the C18 concentrated PVP fraction were separated on analytical TLC plates coated with either C18 reversed phase adsorbant or with silica. One cm sections of the plates were removed, placed in buffer, hydrolyzed, extracted and the p-damascenone charm determined. Standards containing 5 mg each of 1 -O-n-octyl- p-D-glucopyranoside and a-methyl-D-mannoside were run simultaneously on the same plate and visualized by spraying with aniline-diphenylamine-acetone-phosphoric acid 80% (4 mL 4 g 200 mL 30 mL) (10). HPLC separations were performed on a 0.46 mm x 25 cm column packed with 5 im C18, eluted with 60/40 methanol water at a flow of 0.8 mL/min. The refractive index of samples was recorded with a Knauer differential refractometer. Fractions were collected every minute and assayed for p-damascenone charm. 

Methylindole, 22, 94 Methyl isothiocyanate, 21, 81, 83 Methyl isothiourea, reaction of sulfate with hydrazine sulfate, 26, 9 Methyl lactate, 26, 6 6-Methyllepidine, 26, 47 8-MethyUepidine, 26, 47 a-Methyl-d-mannoside, 22, 86 Methyl mercaptan, 26, 9 N-Methyl- (3 methoxy-4-hydroxy-phenyl)-alanine, 22, 91 2-Methyl-6-methoxyqumohne, 26, 47 Methyl myristate, 20, 67 a-Methylnaphthalene, 26, 93 Methyb -octylamine, 25, 19 Methyl palmitate, 20, 67 Methylphenyldiketoke, 23,1 /3-(2-MEIH LPHENYL)-JiTUYLAMlNE, 23, 72... 

All 20 naturally occurring amino acids, N-acetylmannosamine, 2-deoxy-D-glucose, D-fmctose, gentiobiose, GlcNAc, D-glucose, maltose, D-mannitol, D-mannose, a-methyl-D-glucoside, p-methyl-D-glucoside, a-methyl-D-mannoside, D-sorbitol, L-sorbose, sucrose, trehalose, D-) lose... 

Pseudomonas Arbutin, D-lyxose, L-methionine, a-methyl-D-mannoside,... 

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