9- -9H-xanthen

Bengal Rose 3,4,5,7-Tetrachloro-3, 6 -dihydroxy-spiro[isobenzofuran-l(3H), 9 -[9H]xanthen]-3-one] disodium salt... 

The ORAC method was first proposed by Cao and co-workers in 1993. Like in the TRAP method, they used a fluorescent indicator. Determination of antioxidant activity by this method is based on measurement of decreasing fluorescence of the indicator caused by the radicals generated in the system. The reaction mixture in their proposal consisted of a fluorescent indicator p-phycoerythrin (p-PE), 2,2 -azobis(2-amidinopropane) dihydrochloride (AAPH) as a peroxyl radical generator and the analysed sample [42]. Attributing the low purity of p-phycoerythrin (approx. 30%) to the low reproducibility of fluorescence and the occurrence of different forms of phycoerythrin, Ou and co-workers [43] modified the method by replacing the indicator with fluorescein (3, 6 -dihydroxyspiro[isobenzofuran-l[3H],9 [9H]-xanthen]-3-one). 

Fluorescein (FL) (3, 6 -dihydroxyspiro[isobenzofuran-l[3H], 9 [9H]-xanthen]-3-one) was used for the ORAC assay. Excitation and emission filters of 490 and 520 nm, respectively, were used. A stock solution of 1.2 mM FL was diluted to a working solution of 94 nM made up in 0.75 mM phosphate buffer (pH 7.4). Two pipettors, which were part of the microplate... 

SODIUM SODIUM FLUORESCEIN SODIUM FLUORESCEINATE SODIUM SALT of HYDROXY-o-CARBOXY-PHENYL-FLUORONE SOLUBLE FLUORESCEIN SPIRO(ISOBENZOFURr N)-l(3H),9 -(9H)XANTHENE-3-ONE, 3, 6 -DIHYDROXY-DISODIUM SALT URANTN URANINE A EXTRA URANINE USP XII URANINE YELLOW 11824 YELLOW ... 

SPIRO(ISOBENZOFURAN)-l(3H),9 -(9H)XANTHENE-3-ONE, 3, 6 -DIHYDROXY-DISODIUM SALT see FEWOOO... 

Fluorescein sodium, 3, 6 -dihydroxyspiro[isobenzofuran-l(3H),9 -[9H]xanthen], C2oHioNa205, CAS number 518-47-8, is a yellow acid dye of the xanthene series. Its molecular weight is 376 Da, and its solubility in water at 15°C is 50% (i.e., it is freely soluble). It is generally formulated as its sodium salt (Figure 16-1). When exposed to light, fluorescein maximally absorbs light at approximately 493 nm and emits (fluoresces) at approximately 520 nm. Figure 16-2 illustrates the excitation and emission spectra of dilute fluorescein in phosphate buffer. 

Merbromin, < 2r7r D/6roino-3 6-dr hydro y-3-oxospiro(isobenzajuran-l(3H),9 9H]xanthen]-4 -yi)hydr-oxymercury disodium xatt [2 -dibromo-9-io-earhvxyphen ... 

Beilstein Handbook Reference) acid eosin BRN 0063410 Bromeosin Bromoeosin C.l. 45380 2 C.l. Solvent Red 43 CCRIS 4904 Cl 45380 2 D C Red no. 21 D and C Red No. 21 D C Red No. 21 2-(3,6-Dihydroxy-2,4,5,7-tetrabromoxanthen-9-yl)-benzoic acid 3,6-Dihydroxy-2,4,5,7-tetrabromospiro-(xanthene-9,3 -phthalide) EINECS 239-138-3 Eosin Eosin A Eosin acid Eosin 3J Eosin 4J extra Eosin A extra Eosin B Eosin C Eosin DH Eosin G Eosin G Extra Eosin GGF Eosin JJS Eosin KS Eosin yellowish Eosin Y spirit soluble Eosine acid Fluorescein, 2, 4, 5 ,7 -tetrabromo- Japan Red 223 Japan Red No. 223 NSC 244436 Red No. 223 Solvent Red 43 Spira(isobenzofuran-1(3H),9 -(9H)-xanthen)-3-one, 2, 4, 5, 7 -tetrabromo-3 ,6 -di-hydroxy- Water soluble eosin. The alkali salts of tetrabromo-fluoresceine, dyes wool and silk yellowish red used as a microscopic stain and a fluorescent tracer dye red writing ink cosmetic products and a colorant for motor fuel. Red solid. 

Calcodd erythrosine N Canacert erythrasine BS 9-(o-Carboxyphenyl)-6-hydraxy-2,4,5,7-tetraiodo-3H-xanthene-3-one disodium salt monohydrate Caswell No. 425AB CCRIS 892 Cerven kysela 51 Cerven potravinarska 14 Cl 45430 Cilefa Pink B D C Red No. 3 3. 6-Dlhydroxy-2, 4, 5, 7 -tetraiodospiro(iso-benzofuran-1(3H),9 (9H)xanthen)-one disodium salt Disodium 9-(0-carboxyphenyl)-6-hydroxy-2,4,5,7-tetra-iodo-3H-xanthen-3-one monohydrate Disodium 3, 6 -dihydroxy-2, 4, 5, 7 -... 

Carboxyphenyl)-6-hydroxy-3-isoxanthenone 9-(o-Carboxyphenyl)-6-hydroxy-3H-xanthen-3-one C.l. 45350 1 C.l. Solvent Yellow 94 CCRIS 7076 D C Yellow no. 7 3, 6 -Dihydroxyfluoran 3, 6 -Dihydroxyspiro(isobenzofuran-1(3H),9 -(9H)xanthen)-3-one 3,6-Dlhydroxysplro-(xanthene-9,3 -phthallde) EINECS 219-031-8 Fluoran, 3, 6 -dihydroxy- 3,6-Fluorandiol Fluorescein Fluor-escein acid Fluorescein Red Fluoresceine Hidacid fluorescein HSDB 2128 2-(6-Hydroxy-3-oxo-(3H)-xanthen-9-yl)benzoic acid Japan Yellow No. 201 Resorcinolphlhalein Soap Yellow F Solvent Yellow 94 Spiro(isobenzofuran-1(3H),9 -(9H)xanthen)-3-one, 3, 6 -... 

Tetrabromo-3, 6 -dihydroxyspiro [isobenzof uran-1 (3H),9 -[9H] xanthen]-3-one disodium salt Tetrabromofluorescein... 

CAS 596-03-2 EINECS/ELINCS 209-876-0 Synonyms Acid orange 11 4, 5 -Dibromo-3, 6 -dihydroxyspiro [isobenzofuran-1(3H),9 -[9H] xanthen]-3-one disodium salt Classification Xanthene color Empirical C2oHioBr20s Toxicology TSCA listed Uses Colorant in cosmetics Manuf./Distrib. Aldrich http //www.sigma-aldrich.com, Fluka http //www.sigma-aldrich.com, Sigma... 

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