9- -9H-xanthen

SPIRO(ISOBENZOFURAN)-l(3H),9 -(9H)XANTHENE-3-ONE, 3, 6 -DIHYDROXY-DISODIUM SALT see FEWOOO 

Tetrabromo-3, 6 -dihydroxyspiro [isobenzof uran-1 (3H),9 -[9H] xanthen]-3-one disodium salt Tetrabromofluorescein 

Fluorescein (FL) (3, 6 -dihydroxyspiro[isobenzofuran-l[3H], 9 [9H]-xanthen]-3-one) was used for the ORAC assay. Excitation and emission filters of 490 and 520 nm, respectively, were used. A stock solution of 1.2 mM FL was diluted to a working solution of 94 nM made up in 0.75 mM phosphate buffer (pH 7.4). Two pipettors, which were part of the microplate 

Bengal Rose 3,4,5,7-Tetrachloro-3, 6 -dihydroxy-spiro[isobenzofuran-l(3H), 9 -[9H]xanthen]-3-one] disodium salt 

Eosin Yellow 2, 4, 5, 7 -Tetrabromo3, 6 ihydroxyspiro[isobenzofuran 1(3H), 9 -[9H]xanthen-3-one disodium salt 

Tetrachloro-3, 6 -dihydroxy-2, 4, 5, 7 -tetraiodo-spiro[isobenzofuran-1 (3H),9 [9H]xanthen]-3-one dipotas-sium salt. Disodium 3,4,5,6-tetrachloro-2-(2,4,5,7-tetraiodo-6-ox-ido-3-oxoxanthen-9-yl)benzoate EINECS 211-183-3 Flu-orescein, 

Phloxim B 2 , 4 , 5 , 7 -Tetrabromo-4,5,6,7-tetra-chloro-3 , 6 -dihydroxy-spiro [isobenzofuran-1] (3H),9 -[9H]xanthen-3-one-sodium salt 

Carboxyphenyl)-6-hydroxy-3-isoxanthenone 9-(o-Carboxyphenyl)-6-hydroxy-3H-xanthen-3-one C.l. 45350 1 C.l. Solvent Yellow 94 CCRIS 7076 D C Yellow no. 7 3, 6 -Dihydroxyfluoran 3, 6 -Dihydroxyspiro(isobenzofuran-1(3H),9 -(9H)xanthen)-3-one 3,6-Dlhydroxysplro-(xanthene-9,3 -phthallde) EINECS 219-031-8 Fluoran, 3, 6 -dihydroxy- 3,6-Fluorandiol Fluorescein Fluor-escein acid Fluorescein Red Fluoresceine Hidacid fluorescein HSDB 2128 2-(6-Hydroxy-3-oxo-(3H)-xanthen-9-yl)benzoic acid Japan Yellow No. 201 Resorcinolphlhalein Soap Yellow F Solvent Yellow 94 Spiro(isobenzofuran-1(3H),9 -(9H)xanthen)-3-one, 3, 6 - 

SODIUM SODIUM FLUORESCEIN SODIUM FLUORESCEINATE SODIUM SALT of HYDROXY-o-CARBOXY-PHENYL-FLUORONE SOLUBLE FLUORESCEIN SPIRO(ISOBENZOFURr N)-l(3H),9 -(9H)XANTHENE-3-ONE, 3, 6 -DIHYDROXY-DISODIUM SALT URANTN URANINE A EXTRA URANINE USP XII URANINE YELLOW 11824 YELLOW  

Eosin blue Eosine blue Fluorescein, 2, 4, 5, 7 -tetrabromo-4,5,6,7-tetrachloro-, disodium salt Food dye red 104 Food red 104 Japan red 104 Phloxin B Phloxine B Red 104 Spiro [isobenzofuran-1(3H),9 -[9H] xanthen]-3-one, 2, 4, 5, 7 -tetrabromo-4,5,6,7-tetrachloro-3, 6 -dihydroxy-, disodium salt 2, 4, 5, 7 -Tetrabromo-4,5,6,7-tetrachloro-3, 6-dihydroxyspiro [isobenzofuran-1(3H),9 -[9H] xanthen]-3-one, disodium salt Classification Xanthene color Empiricai C2oH2Br4Cl40s 2Na Properties Dk. red powd. sol. 90 mg/ml in water m.w. 829.64 

The ORAC method was first proposed by Cao and co-workers in 1993. Like in the TRAP method, they used a fluorescent indicator. Determination of antioxidant activity by this method is based on measurement of decreasing fluorescence of the indicator caused by the radicals generated in the system. The reaction mixture in their proposal consisted of a fluorescent indicator p-phycoerythrin (p-PE), 2,2 -azobis(2-amidinopropane) dihydrochloride (AAPH) as a peroxyl radical generator and the analysed sample [42]. Attributing the low purity of p-phycoerythrin (approx. 30%) to the low reproducibility of fluorescence and the occurrence of different forms of phycoerythrin, Ou and co-workers [43] modified the method by replacing the indicator with fluorescein (3, 6 -dihydroxyspiro[isobenzofuran-l[3H],9 [9H]-xanthen]-3-one). 

---