2D-protein map

Colloidal Gold Staining and Immunodetection in 2D Protein Mapping... 

Protein fingerprinting (e.g., 2D protein map) Species, strain Abdel-Aty (1991), Kobi, Zugmeyer, Potier, and Jaquet-Gutfreund (2004)... 

Moreover, experimental reference maps of human tissues were studied p7 and Mr coordinates of identified spots were retrieved from the SWISS-2D-PAGE database, the values <3X = 0.009 pH and av = 0.0002 log Mr were assumed for spot dimension since they represent the standard case for experimental 2D-PAGE maps—normal sample loading of a tissue homogenate (ca. 1 mg total protein) and standard gel sizes (18 x 20 cm, IEF x SDS-PAGE). 

It must be emphasized that the availability of the SMO and 2D autocovariance function methods as two independent statistical procedures to estimate the same parameter, in, the number of proteins, is a helpful tool to verify the reliability of the results obtained. In the case of the 2D PAGE map of colorectal adenocarcinoma cell line (DL-1) an excellent agreement was found between the values obtained from the SMO method—m = 101 10 and m = 105 10—and the 2D autocovariance function procedure—m = 104 10 (Pietrogrande et al., 2006a). 

A 2D liquid mass mapping method has been developed in our laboratory for the analytical profiling of proteins in complex biological material. In the present study, we demonstrate the capability of this method for comparative protein mapping of isogenic... 

FIGURE 4.1 2D polyacrylamide gel electrophoresis (2D-PAGE) maps of protein mixtures. (See text for full caption.)... 

First Siena conference. 2D Electrophoresis From Protein Maps to Genomes, 5-7 September 1994. 

Initial results with LCxLC-MS in the analysis of proteins were reported by Opiteck et al. [73-74], using either ion-exchange (lEC) or size-exclusion (SEC) chromatography in the first dimension and RPLC in the second dimension. Protein mapping similar to 2D-GE was demonstrated, but with inferior efficiency. 

Conventional methods for the study of protein phosphorylation rely on radioactive labelling, 2D-GE protein mapping, and Edman degradation. Early studies in LC-MS characterization of protein phosphorylation involve MS-MS analysis of modified tryptic peptide to determine the phosphorylation site by complementary peptide mapping, e.g., [5-7]. In the LC-MS analysis of tryptic and V8-protease digests of a phosphorylated (ppl9) and nonphosphorylated (pl9) 19-kDa cytosolic protein, two sets of ions with a phosphate-characteristic mass difference of 80 Da were observed. Sequence analysis of the relevant peptides by MS-MS showed that phosphorylation occurs at Ser-25 and Ser-38 [7]. 

D gel DEHP decanoic acids, PPAR Rat liver Protein-mapping algorithm... 

D-MS Nicotine Rat blood 2D image protein-mapping proteins algorithm fuzzy logic... 

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