Zone clearing analysis

The results obtained from this large zone SEC analysis clearly define the stoichiometry of the self-association of Enzyme I as a monomer-dimer reaction. Furthermore, this approach provides a sieans of determining the effects of temperature, Mg, and phosphoenolpyruvate on this self-association. These results are consistent with the proposed model for the regulation of the PTS through Enzyme I. 

While it is generally acknowledged therefore that MDGC can increase resolution by transfering zones from one column to another, there is clearly a limited opportunity to improve the total analysis through this approach. 

It is again clear that the two benefits of increased sensitivity and better resolution are both achieved, where these arise from zone compression and phase selectivity, respectively. However, since this mode of analysis is relatively new, it has yet to be tested for a wide range of applications such studies will be required to fully demonstrate its general utility. It is unclear whether this operational mode of selective MDGC constitutes a mode which is consistent with the definition of comprehensive... 

Fungicidal activity was determined by the disc method and zones of inhibition were recorded by measuring the diameter (mm) of the inhibition zone. Yeast cultures (S. cerevisiae) showed growth inhibition (clear area surrounding disc) by sediment extracts from all stations when compared to control discs. HPLC analysis of sediment extracts showed more than 20 components in the migration profile of each station. Of these components, a fraction demonstrated to possess cytolytic activity in the crude extract (Ve = 24 ml) was present in all stations when compared to the migration profile of the active fraction (Figure 2). 

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