Zebrafish mutations

Zebrafish Brachydanio/Danio rerio) embryos can be produced in large numbers and carry sufficient nutrients within the egg sack to allow development within micro plate volumes [52, 53]. Mutation frequency has been estimated indirectly using transgenic fish [54[ and UDS, comet, MNT and alkaline filter elution methods have been used effectively [55]. At present, there has been insufficient study of the model to understand predictivity of mammalian genotoxicity, and none of the methods has been demonstrated at throughputs sufficient for hit and lead screening. 

Amanuma, K., Takeda, H., Amanuma, H. and Aoki, Y. (2000) Transgenic zebrafish for detecting mutations caused by compormds in aquatic environments. Nature Biotechnology, 18, 62-65. 

From phenotype to gene mapping of mutations in the zebrafish... 

Ghosh, S., Moore, S., Bell, R. M., and Dush, M. (2003). Functional analysis of a phosphatidic acid binding domain in human Raf-1 kinase Mutations in the phosphatidate binding domain lead to tail and trunk abnormalities in developing zebrafish embryos. J. Biol. Chem. 278, 45690-45696. 

Types of mutation generated by commonly used mutagenic agents in zebrafish... 

Grunwald, D.J. and G. Streisinger. Induction of mutations in the zebrafish with ultraviolet light. Genet. Res. 59 93-101, 1992. 

Begemann, G., T. Schiling, G.J. Rauch, R. Geisler and P. Ingham. The zebrafish neckless mutation reveals a requirement for Raldh2 in mesodermal signals that pattern the hindbrain. Development 128 3081—3094, 2001. 

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