Yeast gene annotation

The commercial success of the spotted microarray, like that of Affymetrix s GeneChip, is content-driven. In order to provide customers with comprehensive gene expression microarray products, manufacturers must obtain gene-specific annotated sequences covering genomes of major interest to the scienhfic community (e.g., genomes of humans, yeasts, and mice). 

The presence of the second active form of MIPS with a —65 kDa subunit indicated that the inositol requirement of the organism might be provided by the interplay of two different MIPS enzymes, probably by differential expression through time and space. The results indicate that the —65 kDa MIPS protein of Synechocystis might be coded by the ORF sill981, annotated as a putative acetolactate synthase (unpublished data from this laboratory). This gene also functionally complements the inositol auxotrophic yeast strain FY250 and Schizosaccharomyces pombe, a natural inositol auxotroph, and the expressed protein is immunoreactive to anti MIPS antibody. 

---