Yeast artificial chromosome vector

For repetitive DNA sequences, such as alphoid repeats, especially for identification of centromeric regions on chromosomes, as well as in mterphase nuclei, I routinely prepare labeled probes by PCR using a centromeric DNA-specific set of primers. For single-copy gene mapping by FISH, it is essential to use DNA cloned into either cosmids or in yeast artificial chromosome vectors (YAC) m order to obtain the level of sensitivity that will enable visualization of signal with a fluorescence microscope... 

Burke D T, Olson M V (1991). Preparation of clone libraries in yeast artificial-chromosome vectors. Methods Enzymol. 194 251-270. 

Eig. 5. Restriction map of the yeast artificial chromosome (YAC) vector used for cloning very large fragments of eukaryotic DNA. Terms defined in text... 

A Bacterial Artificial Chromosome (BAC) is a vector that allows the propagation of larger exogenous DNA fragments, up to several hundred kb. BACs are propagated in recombination-deficient strains of E. coli. They are more stable and easier to handle than yeast artificial chromosomes (YACs). 

An yeast artificial chromosome (YAC) is a vector that allows the propagation of large exogenous DNA fragments, up to several megabases, in yeast. 

Yeast artificial chromosome (YAC) Large segments of DNA (up to 1 million bases) from another species spliced into DNA of yeast. The new construct that carries the foreign DNA is called a vector. 

B12. Burke, D. T., Carle, G. F., and Olson, M. V., Cloning of large segments of exogeneous DNA into yeast by means of artificial chromosome vectors. Science 236, 806-812 (1987). 

Research work with large genomes and the associated need for high-capacity cloning vectors led to the development of yeast artificial chromosomes (YACS Fig. 9-8). YAC vectors contain all the elements needed to maintain a eukaryotic chromosome in the yeast nucleus a yeast origin of replication, two selectable markers, and specialized sequences (derived from the telomeres and centromere, regions of the chromosome discussed in Chapter 24) needed for stability and... 

Cloning vectors include plasmids, bacteriophages, and, for the longest DNA inserts, bacterial artificial chromosomes (BACs) and yeast artificial chromosomes (YACs). 

Maynard Olson and colleagues at Washington University invented yeast artificial chromosomes, or YACs, expression vectors for large proteins. 

Various bacterial plasmids, bacteriophages, and yeast artificial chromosomes are used as cloning vectors (Brown, 2001). At the heart of the general approach to generating and propagating a recombinant DNA molecule is a set of enzymes which synthesize, modify, cut, and join DNA molecules. 

Alternative cloning vectors have been developed they can be used for genomic fragments of up to 2 mb for the yeast artificial chromosomes (YAC) (Slow et al.,... 

Figure 6.21. Diagram of a Yeast Artificial Chromosome (YAC). DNA inserts as large as 1000 kb can be propagated in this vector.

Figure 5.16 Diagram of a yeast artificial chromosome (YAC). These vectors include features necessary for replication and stability in yeast ceils.

Yeast artificial chromosome 1111 Yeast cell surface display 1112 Yeast expression vector 1028 Yeast-2-hybrid system 1161 Yeast signal trap 109 Yttrium-90 Zevalin 456, 480 Yttrium-DOTALA.N 518 Yttrium-DOTATATE 518 Yttrium-DOTATOC 516... 

DNA fragments up to 10 base pairs long can be cloned in yeast artificial chromosome (YAC) vectors. 

Yeast artificial chromosome (YAC) A genomic cloning vector which is propagated in yeast as an autonomously replicating chromosome. 

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