Whole blood tube additives

Whole blood/plasma tubes K2EDTA (dry additive) K3EDTA (liquid additive) Na2EDTA (dry additive) Lavender Lavender... 

Whole Blood. It is recommended that the samples be cautiously handled from the start of the collection to maintain integrity and preclude the possibility of contamination, tampering, or mislabeling. All samples should be collected under the close supervision of a health care provider/ physician, and if possible, witnessed by an unbiased observer. Samples should be obtained as soon as possible following the suspected exposure. Additional follow-up samples may be obtained. Blood should be collected in 5 or 7 mL blood tubes. Specific methods may require specific types of tubes, such as purple-top (EDTA) tubes for plasma, or the red-top mbes for serum and vacuum-fill only (unopened). 

Pipet 200 /whole blood into a 4 mL acid-washed polystyrene tube. To make a chemical blank 200 fiL of water are pipetted instead of blood. If the calibration is performed by the internal standard addition technique, it is important to pipet exactly 200 juL of blood. This may achieved by the "reverse pipetting technique". 

A. Specific levels. The whole-blood lead level is the most useful indicator of lead exposure. Relationships between blood lead levels and clinical findings have generally been based on subacute or chronic exposure and not on transiently high values that may result immediately after acute exposure. In addition, there may be considerable interindividual variability. Note Blood lead samples must be drawn and stored In lead-free syringes and tubes ( trace metals tube or royal blue stopper tube containing heparin or EDTA). 

Prepare in water two dilutions of standard preparation containing 2-0 and 14 units per ml together with two similar dilutions of the unknown. Place 1 ml of each dilution into a 6" x test-tube followed by a quantity of thrombokinase solution (about 0-2 ml) sufficient to cause coagulation in the 2u/ml standard tube in about ten minutes after the addition of 1 ml of sulphated whole blood. Add 1 ml of sulphated whole blood to each tube, mix gently avoiding the formation of air bubbles. Record to the nearest fifteen seconds the time for the formation of a firm clot which remains in the bottom of the tube when it is inverted. 

The smallest vertebrates are homogenized whole and most laiger animals can be dissected and different tissues removed for storage in 1.5-ml Eppendorf microtubes at — 70° until homogenized. Heart, kidneys, or liver are sufficient to score more than 20 proteins testes, spleen, brain, and muscle can be sampled for tissue-specific enzymes. Additionally, blood, muscle, and/or saliva from most vertebrates can be conveniently sampled without killing the animal. During all sample preparation steps, keep tubes on crushed ice. 

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