Vortex-glass

We have studied both static and dynamic properties of the vortex systems, such as Abrikosov vortex lattice formation, vortex lattice melting, the KT transition, and the vortex glass transition, from the point of view of an analogy between our system and colloid, polymer systems [3]. Our study is based on computer simulations of two efficient model equations, recently developed by us. 

Flow measurements using nonintrusive or low mechanical ac tion principles are desired, such as magnetic, vortex-shedding, or Coriolis-type flowmeters. Orifice plates are easy to use and reliable but have a limited range and may not be suitable for streams which are not totally clean. Rotameters with glass tubes should not be used. 

Add 20 mL of MeOH-30mM NaOAc solution (4 1, v/v NaOAc adjusted to pH 2.5-3) to the soil, and then the mixture should be vortex mixed for 30 s, sonicated for 5 min, vortex mixed momentarily, and centrifuged for 5 min at 2500 rpm. Decant the supernatant into a clean lOO-mL graduated cylinder. Repeat the first two steps twice for a total of 60 mL of extraction solution used (dilute to 60 mL with MeOH as necessary). Transfer the solution into a glass, capped bottle (pour back and forth twice to mix). 

The vortex flow reactor was a glass Couette cell driven by a Bruker RheoNMR system. The cell consisted of a stationary outer glass tube with an id of 9 mm and a rotating inner glass tube with an od of 5 mm, giving a gap of 2 mm. The Couette was filled with cylindrical bacterial cells, F. nucleatum ( 2 x 20 pm), suspended in water at a concentration of =10" cells mL-1. 

Cells are broken by adding 4 mm diameter acid-washed glass beads (Thomas Scientific) equal to one-half of the total volume of resuspended cells and rigorously vortexed for 30 s, followed by 1 min on ice, conducted 5 times. Less than 5 cycles were not sufficient for complete cell lysis, whereas more than 5 cycles of vortexing resulted in a significant loss of co-purifying components. 

Add 1.5 ml of chilled glass beads (0.45-0.55 mm) and vortex vigorously in a bead beater by two pulses of 90 s at maximum level to achieve complete lysis. 

Lipid extraction 200 pL of plasma sample is mixed by brief vortexing with 200 pL of ethanol followed 1 min of vortexing with 800 pL hexane. After centrifugation for 5 min at 5000 rpm, 400 pL of upper (hexane) phase is transferred to a glass tube with screwed cap, dried under nitrogen, and kept at - 60°C until PCL analysis. For PCL analysis the sample is dissolved in 400 pL of MeOH by 30 s of vortexing, and 100 pL aliquots are taken for ACL assay. 

Cyclosporine D (200 fxg/L) in methanol and ZnS04 (50 g/L) aqueous solution (1 1 v/v) served as the IS. The IS (1.5 mL) was accurately transferred into disposable glass tubes (13 x 100 mm), mixed with 0.5 mL calibration standard, control, or patient sample by vortexing for 30 sec, and centrifuged at 500 g for 2 min. C8 cartridges (conditioned by 1 mL acetonitrile followed by 0.5 mL deionized water) were loaded with whole blood supernatants followed with 1 mL acetonitrile/water wash solution (2 3 v/v), then loaded into the AASP. 

Total protection from liquids and vapors can be provided by an impervious hood incorporating a glass or plastic window. Unfortunately, these are very hot and may require additional ventilation directly from an air line, or perhaps through a device known as a vortex tube that converts supplied compressed air to either a cool or warm flow as required. 

In the method, soil samples are extracted by shaking or vortexing with the solvent. Water samples are extracted by shaking in a separatory funnel. If there is a potential for the presence of compounds that interfere with the method and make the data suspect, silica gel can be added to clean the extract. Sample extract aliquots are placed close to the bottom of a glass plate coated with a stationary phase. The most widely used stationary phases are made of an organic hydrocarbon moiety bonded to a silica backbone. 

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