Glass beads washing

Once separated, the supports are washed using acid (HCl) and sdanized, ie, treated with dimethyl dichi orosilane [75-78-5] (DMCS), to reduce the polarity. Sdanizing replaces adjacent SiOH groups with nonpolar CH caps. In addition to diatomaceous earths, supports of carbon (qv), halocarbons, eg. Teflon, and glass beads are in use by various chromatographers. 

In vivo spectra of complexed tin on Pseudomonas 244 are essentially Identical to those of the glass bead model system, exhibiting an emission maxlmvm at 475 nm. A lower Intensity residual peak at 550 nm In Figure 4 Is due to uncomplexed flavonol, which was not completely removed from the cell membrane despite multiple washings. 

Ethylene Dibromide. Technical material was washed three times with concentrated sulfuric acid, rinsed with water, and dried over calcium chloride. It was then distilled through 1 foot of glass beads. The fraction boiling between 130.2° and 131.2° C. was used. Every third day an aqueous solution was prepared by weighing approximately 0.5000 gram of ethylene dibromide into 2000.0 grams of water. It was stored in a dark bottle to minimize light decomposition. 

Cells are broken by adding 4 mm diameter acid-washed glass beads (Thomas Scientific) equal to one-half of the total volume of resuspended cells and rigorously vortexed for 30 s, followed by 1 min on ice, conducted 5 times. Less than 5 cycles were not sufficient for complete cell lysis, whereas more than 5 cycles of vortexing resulted in a significant loss of co-purifying components. 

The Ballotini glass beads (-50 pm diameter) used in these experiments should be cleaned in dilute NaOH solution, washed thoroughly with... 

This test is to assess the efficacy of the vial/ampoule washer in reducing the particulate level in contaminated vials. Particulate matter to be determined in eight vials/ampoules, each spiked with approximately 500 particles of 40- im glass beads. A sample is to be taken from each individual needle at the end of vial/ampoule washing at set operational parameter of vial/ampoule washer and analyzed to determine the reduction in particulate level. The X ml vials/ampoules are to be washed according to the operational parameters described for each vial/ampoule size. The results are to be compared with negative and positive controls. The test results for each size of vial/ampoule are to be entered in Table 2. 

Both enzyme-coated glass beads can be recycled. Beads have to be washed five times with 1 ml reaction buffer. Washed beads can be stored in reaction buffer at 4°C. 

Acid-washed glass beads, G1152 (Sigma-Aldrich, Poole, UK). 

Add acid-washed glass beads and 1 mL of PB to the flask and incubate for 1 h at 37°C with agitation (220 rpm) to resuspend the lipid layer. 

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