Vibrio cholerae, neuraminidase

Viral replication, packaging and release. Drug Disc Today 1 388-397 Meindl P, Tuppy H (1969) 2-Deoxy-2,3-dehydrosialic acids. II. Competitive inhibition of Vibrio cholerae neuraminidase by 2-deoxy-2,3-dehydro-V-acyIneuraminic acids. Hoppe-Seyler s Z Physiol Chem 350 1088-1092... 

Fig. 4.—Mass Fragmentography (m/e 161) of Methylated Hexose Derivatives from the Disialosy] Ganglioside. [Top, before treatment with neuraminidase bottom, after treatment with Vibrio cholerae neuraminidase. The methylated hexose derivatives identified are (A) 2,4,6-tri-O-methylgalactose (B) 2,3,6-tri-O-methylglucose (C) 2,3,4,6-tetra-O-methylgalactose. The peaks eluting before C are unrelated signals that were also detected in a blank experiment employing the neuraminidase treatment. Conditions 3% of QF-1, at 190°. Reproduced, by permission, from Ref. 80.]...

Add 0.05 units of Vibrio cholerae neuraminidase and 5 units of galactose oxidase per milliliter of cell suspension. 

Colostrum is a richer source of these sialic acid derivatives than milk, and it was from cow colostrum that JV-acetyl-O-acetylneuraminolactose was isolated in about 0.025% yield however, owing to the extreme lability of the 0-acetyl group, most of the subsequent work has been performed on the A "-acetylneuraminolactose. This compound has been split by influenza virus and Vibrio choleras neuraminidase into lactose and A -acetylneura-... 

S. Crennell, E. Garman, G. Laver, E. Vimr, and G. Taylor, Crystal straeture of Vibrio cholerae neuraminidase reveals dual leetin-like domains in addition to the catalytic domain, Structure, 2 (1994) 535-544. 

Scheme 6. Disaccharide synthesis of NeuSAc derivatives by catalysis with vibrio cholerae neuraminidase.

Table 2. Enzymatic glycosylation using vibrio cholerae neuraminidase )...

Competitive inhibition of Vibrio cholerae neuraminidase by 2-deoxy-2,3-dehydro-A/-acyl-neuraminic acids. Hoppe-Seylers Zeitschrift Fur Physiologische Chemie,... 

Luo Y, Li SC, Chou MY, Li YT, Luo M (1998) The crystal structure of an intramolecular trans-sialidase with a NeuAc alpha2->3 Gal specificity. Structure 6 521-530 Moustafa I, Connaris H, Taylor M, Zaitsev V, Wilson JC, Kiefel MJ, von Itzstein M, Taylor G (2004) Sialic acid recognition by Vibrio cholerae neuraminidase. J Biol Chem 279 40819 0826... 

Controls included i) section pretreatment with Vibrio cholerae neuraminidase for the specificity of LFA reactivity (indicating strong general sialomucin expression), ii) section pretreatment with Newcastle disease virus to check for the specificity of MAA binding (indicating sialomucins characterized by 2,3 bound sialic acid), iii) lectin pretreatment with... 

Glycoproteins containing sialic acid may be detected by staining with a cationic carbocyanine dye before and after digestion with neuraminidase a change in the colour of the stain from blue to red-purple indicates the presence of sialic acid. The n.m.r. spectra of iV-acetylneuraminic acid and its methyl ester in DMSO and in water have indicated that the acid exists predominantly in the /S-form in solution. The finding supports the assumption that 7V-acetyl-neuraminic acid, produced by the enzymic cleavage of its a-ketosides, leaves the catalytic site of Vibrio cholerae neuraminidase as the j3-anomer. 

Vibrio cholerae neuraminidase was activated by divalent cations, and the effects of these ions were thoroughly investigated. The effect of V. cholerae neuraminidase on the uptake of 5-hydroxytryptamine by human platelets has been studied. ... 

Khorlin, A. Y, Privalova, I. M., Zakstelskaya, L. Y, Molibog, E. V., and Evstigneeva, N. A., 1970, Synthetic inhibitors of Vibrio cholerae neuraminidase and neuraminidases of some influenza virus strains, FEES Lett. 8 17-19. 

Portoukalian, J., and Bouchon, B., 1986, Hydrolysis of all gangliosides, including GMl and GM2, on thin-layer plates by Vibrio cholerae neuraminidase, J. Chromatogr. 380 386-392. 

Vimr, E. R., Lawrisuk, L., Galen, J., and Kaper, J. B., 1988, Cloning and expression of the Vibrio cholerae neuraminidase gene nanH in Escherichia coli, J. Bacteriol. 170 1495-1504. 

Holmquist, L., and Brossmer, R., 912a. On the specificity of neuraminidase. The carboxymethyl a-ketoside of N-acetyl-D-neuraminic acid, a Vibrio cholerae neuraminidase substrate having two anionic sites, FEBS Lett. 22 46. 

Carmody, P. J., and Rattazzi, M. C., 1974, Conversion of human hexosaminidase A to hexosaminidase B by crude Vibrio cholerae neuraminidase preparations Merthiolate is the active factors, Biochim. Biophys. Acta 371 117. 

Barton, N. W., and Rosenberg, A., 1973, Action of Vibrio cholerae neuraminidase (sialidase) upon the surface of intact cells and their isolated sialolipid components, J. Biol. Chem. 248 7353-7358. 

Bekesi, J. G., St. Arneault, G., and Holland, J. F., 1971, Increase of leukemia L1210 immunogenicity by Vibrio cholerae neuraminidase treatment, Cancer Res. 31 2130. 

Ray, P. K., and Simmons, R. L., 1973, Differential release of sialic acid from normal and malignant cells by Vibrio cholerae neuraminidase or influenza virus neuraminidase. Cancer Res. 33 936-939. 

Crennell, S Carman, E Laver, G Vimr, E Taylor, G. Crystal structure of Vibrio cholerae neuraminidase reveals dual lectin-Uke domains in addition to the catalytic domain Structure, 1994, 2(6), 535-44. 

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