Vegetative culture medium

Vegetative culture medium (Soluble starch, Peptones, Beef extract, Sodium chloride. Yeast extract, water)... 

Employing aseptic techniques, the inocolum obtained is used to inoculate a 2 L Erlenmeyer flask containing a 500 ml portion of a sterilized vegetative culture medium having the following composition Soluble starch 10 g, Peptones 5 g, Beef extract 5 g, Sodium chloride 5 g, Yeast extract 2.5 g and tap water 1100 ml. 

The SF-837 strain, namely Streptomyces mycarofaciens identified as ATCC No. 21454 was inoculated to 60 liters of a liquid culture medium containing 2.5% seccharified starch, 4% soluble vegetable protein, 0.3% potassium chloride and 0.3% calcium carbonate at pH 7.0, and then stir-cultured in a jar-fermenter at 28°C for 35 hours under aeration. The resulting culture was filtered directly and the filter cake comprising the mycelium cake was washed with dilute hydrochloric acid. 

The presence of iron in vegetable ash has been known since the beginning of the eighteenth century. Although iron is not a constituent of the chlorophyll molecule, a plant grown in a culture medium entirely free from it produces no chlorophyll. According to Roscoe W. Thatcher, plants take iron from the soil in the smallest proportion of any of the essential elements. Since ferrous compounds are toxic to plants, only the soluble ferric compounds can be utilized (195). 

Two 300 ml Erlenmeyer flasks, each containing 60 ml of the following culture medium for the vegetative phase, were prepared peptone 0.6% dry yeast... 

Microbiological Sampling. Vegetative Cells. Cellular samples were obtained at various times after inoculation by sampling a known aliquot of the culture medium. These samples were centrifuged (Sorvall Superspeed RC 2-B, 7000 rpm) at 6°C for 10 min. The supemate was... 

Flasks shaken with lOOmL of medium were inoculated with 1 % of a vegetative culture. After 24 h 50 mg of the substrate in 1 mL of EtOH are added. Incubation at 28 =C for 3 d. The reaction mixture is extracted with four 200-mL portions of CH2CI,. Further purification is achieved with preparative TLC. 

The culture medium is heated up in circulating steam at 100 °C for a sterilizing time of 30 minutes on three consecutive days. Between the individual heating periods, the culture medium is incubated at about 25 C. When the culture medium is heated up to 100 C, the vegetative cells are killed. Spores of bacteria and moulds, however, survive a single treatment at 100 °C. This is why the intermediate periods of incubation are introduced. Vegetative forms develop from the steam-resistant spores and these are then killed by the subsequent heating period. The heat treatment is carried out three times to increase the reliability of the process. 

Flax is a temperate climate plant the best weather conditions for flax are high temperature and high relative air humidity. The sum of rainfalls during the vegetation period should lie within 110-130 mm (600-800 mm aimually). Flax consumes 400-600 ml of water to produce 1 g of dry matter. The best soils for flax cultivation are loess soils in good culture, medium-heavy, clay-sandy and sand-loamy soils. The flax should be cultivated on soils of good structure, with the ability to hold water and release it in dry conditions. The best soils for flax cultivation are the soil of find and Illrd class, sometimes IVa [5]. 

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