Streptomyces mycarofaciens

The SF-837 strain, namely Streptomyces mycarofaciens identified as ATCC No. 21454 was inoculated to 60 liters of a liquid culture medium containing 2.5% seccharified starch, 4% soluble vegetable protein, 0.3% potassium chloride and 0.3% calcium carbonate at pH 7.0, and then stir-cultured in a jar-fermenter at 28°C for 35 hours under aeration. The resulting culture was filtered directly and the filter cake comprising the mycelium cake was washed with dilute hydrochloric acid. 

Streptomyces mycarofaciens Midecamycin Streptomyces nodosus Amphotericin B Streptomyces noursei Nystatin... 

O Hara, CR Hutchinson. A macrolide 3-O-acyltransferase gene from the mideca-mycin-producing species Streptomyces mycarofaciens. JBacteriol 174 5141-5144, 1992. 

Macrolide antibiotic from cultures of Streptomyces mycarofaciens. Midecamycin A2, A3 and A4 exist also in small amounts in the complex beside main component Midecamycin. 

Hara, O., and C.R, Hutchinson (1990). A macrolide 3-O-acyllransferase gene from the midecamy-cin-producing Streptomyces mycarofaciens. J. Bacteriol. 174 5141-5144. 

Hara, O., and Hutchinson, C. R. (1990). Cloning of midecamycin (MLS)-resistance genes from Streptomyces mycarofaciens, Streptomyces lividans and Streptomyces coelicolor A3(2). J. Anti-biot. 43,977-991. 

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