Vegetative culture medium Soluble starch

Vegetative culture medium (Soluble starch, Peptones, Beef extract, Sodium chloride. Yeast extract, water)... 

Employing aseptic techniques, the inocolum obtained is used to inoculate a 2 L Erlenmeyer flask containing a 500 ml portion of a sterilized vegetative culture medium having the following composition Soluble starch 10 g, Peptones 5 g, Beef extract 5 g, Sodium chloride 5 g, Yeast extract 2.5 g and tap water 1100 ml. 

The SF-837 strain, namely Streptomyces mycarofaciens identified as ATCC No. 21454 was inoculated to 60 liters of a liquid culture medium containing 2.5% seccharified starch, 4% soluble vegetable protein, 0.3% potassium chloride and 0.3% calcium carbonate at pH 7.0, and then stir-cultured in a jar-fermenter at 28°C for 35 hours under aeration. The resulting culture was filtered directly and the filter cake comprising the mycelium cake was washed with dilute hydrochloric acid. 

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