CDNA library construction vector ligation

The use of free primers requires that the double-stranded form of the cDNA synthesized be subsequently ligated to a linearized vector by one of the following three methods [see Fig. 6.6(1)]. The first is a direct, low efficiency blunt-end ligation between double-stranded cDNA and a blunt-ended vector. This is the most straightforward method to clone cDNA from abundant mRNA species and is not suitable for cDNA library construction. 

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