Vasopressin 2- acetic acid

Examples of the way these two entirely different approaches compliment each other are developing in conformation studies with the hormones angiotensin, oxytocin, and vasopressin. In 1964 Craig, Harfenist, and Paladini (7) published the comparative half-escape times shown in Table I in 0.01N acetic acid and another series in Table II using a different membrane less porous and not as selective as the first. Oxytocin and vasopressin are cyclic octapeptides with an S-S linkage closing the ring at the 1-6 positions (5, 6). Their size is thus limited except for the side... 

Oxytocin and vasopressin cannot easily be separated from the common aliphatic amino acids on Sephadex G-25 in spite of a considerable difference in molecular size. In a solvent consisting of acetic acid, pyridine, and water (60 15 25 parts by volume) the separation is an easy matter. The explanation is very simple. The gel is not capable of extensive swelling in this solvent and consequently the pores are smaller and the peptides are therefore excluded. This technique has also been used to separate a- and /3-meIanophore-stimulating hormones of the hypophysis (Porath and Schally, 1962). Gel filtration in mixed volatile solvents provides a convenient way to desalt peptide mixtures a problem often difficult to resolve. 

Vasopressin and oxytocin, tripeptide amides Butanol-acetic acid-water (4.1 1) UV 51... 

The aqueous acetic acid extract possesses three hormonal activities ocytocic, vasopressic, and antidiuretic. In addition, it contains unrelated tissue constituents. Three different techniques have been employed to separate ocytocin and vasopressin and remove the proteins from the extract. 

The drug candidate OPC-51803 is the first nonpeptide vasopressin V2-receptor-selective agonist that is in phase II clinical trials and the best chiral reduction to the intermediate acid (E) was with ruthenium acetate-[(5)-H8-BlNAP] that gave a 77 % ee (Figure 1.10). An improved catalyst system will be needed for large scale production. 

Desmopressin acetate is a synthetic analogue of the natural pituitary hormone 8-arginine vasopressin, an antidiuretic hormone affecting renal water conservation. It contains 1.5 mg/mL desmopressin acetate in a pH-adjusted aqueous solution with hydrochloric acid to 4.0 ... 

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