Variable circular dichroism

Polarization and dichroism methods, such as variable circular dichroism (VCD) and magnetic circular dichroism (MCD), are used to determine band assignments in complex molecules. By using various models, polarization features can be correlated with the observed positions of bands in the near-infrared spectra. Absorption bands are assigned to short-axis polarized Q transitions measured using such techniques. [Pg.34]

Variable-Temperature Magnetic Circular Dichroism Studies of Metalloproteins... [Pg.326]

Probing Metalloproteins Electronic absorption spectroscopy of copper proteins, 226, 1 electronic absorption spectroscopy of nonheme iron proteins, 226, 33 cobalt as probe and label of proteins, 226, 52 biochemical and spectroscopic probes of mercury(ii) coordination environments in proteins, 226, 71 low-temperature optical spectroscopy metalloprotein structure and dynamics, 226, 97 nanosecond transient absorption spectroscopy, 226, 119 nanosecond time-resolved absorption and polarization dichroism spectroscopies, 226, 147 real-time spectroscopic techniques for probing conformational dynamics of heme proteins, 226, 177 variable-temperature magnetic circular dichroism, 226, 199 linear dichroism, 226, 232 infrared spectroscopy, 226, 259 Fourier transform infrared spectroscopy, 226, 289 infrared circular dichroism, 226, 306 Raman and resonance Raman spectroscopy, 226, 319 protein structure from ultraviolet resonance Raman spectroscopy, 226, 374 single-crystal micro-Raman spectroscopy, 226, 397 nanosecond time-resolved resonance Raman spectroscopy, 226, 409 techniques for obtaining resonance Raman spectra of metalloproteins, 226, 431 Raman optical activity, 226, 470 surface-enhanced resonance Raman scattering, 226, 482 luminescence... [Pg.457]

Circular dichroism studies show that up to 50% of EPO s secondary structure is a-helical. The predicted tertiary structure is that of four anti-parallel helices formed by variable-sized loops, similar to many other haemopoietic growth factors. [Pg.265]

Mayer, B. Zhang, X. Nau, W.M. and Marconi, G. (2001) Co-conformational Variability of Cyclodextrin Complexes Studied by Induced Circular Dichroism of Azoalkanes, J. Am. Chem. Soc. 123, 5240-5248. [Pg.216]

Variable temperature magnetic circular dichroism (MCD) studies have revealed similar electronic and magnetic properties for the nickel centres in Jack bean and Klebsiella aerogenes urease. Native and 2-mercaptoethanol and acetohydroxamic inhibited forms of both enzymes have been investigated and in each case the energy of the temperature dependent MCD for the nickel(II) d-d transitions indicates octahedral coordination with mainly oxygen donors. [Pg.112]

In order to understand the charge transfer features of the Blue Copper site, the variable-temperature optical absorption, room-temperature circular dichroism, and magnetic circular dichroism spectra of plastocyanin, stellacyanin, and azurin were studied355. As can be seen for plastocyanin in Fig. 12, the relative intensities (and signs, in the case of CD and MCD) of these transitions vary among the different types of spectra. This is a result of the difference in selection rules for absorption, CD, and MCD spectra, as mentioned in the Introduction. A careful comparison of the three types of spectra and the absorption bandshape temperature dependence (see moment analysis in Ref. 35, pp. 176-177)... [Pg.17]

Manavalan P., Johnson W.C. Jr. (1987) Variable Selection Method Improves the Prediction of Protein Secondary Structure from Circular Dichroism Spectra, Anal. Biochem. 167, 76-85. [Pg.293]

Yoon J, Mirica EM, Stack TDP, Solomon El. Variable-temperature, variable-field magnetic circular dichroism studies of tris-hydroxy-and ti(3)-oxo-bridged trinuclear Cu(II) complexes Evaluation of proposed structures of the native intermediate of the multicopper oxidases. J. Am. Chem. Soc. 2005 127 13680-13693. [Pg.1403]