UTP

P2Y55 and P2Y6 receptors. UTP (48) is equipotent with ATP at the P2Y2 i ceptor. 

FIGURE 23.18 The UDP-glucose pyrophosphorylase reaction is a phosphoanhydride exchange, with a phosphoryl oxygen of glu-cose-l-P attacking the m-phosphorus of UTP to form UDP-glucose and pyrophosphate. 

The reaction proceeds via attack by a phosphate oxygen of glucose-l-phosphate on the a-phosphorus of UTP, with departure of the pyrophosphate anion. The reaction is a reversible one, but—as is the case for many biosynthetic reactions —it is driven forward by subsequent hydrolysis of pyrophosphate ... 

Figure 25.7 Glycoprotein formation occurs by initial phosphorylation of the starting carbohydrate to a glycosyl phosphate, followed by reaction with UTP to form a glycosyl uridine 5 -diphosphate. Nucleophilic substitution by an -OH (or -NH2) group on a protein then gives the glycoprotein.

P2Y2 Immune cells, epithelial and endothelial cells, kidney tubules, osteoblasts UTP= ATP, UTPyS, INS 37217, INS 365 Suramin > RB2, AR-C126313 Gq/Gn and possibly Gj PLC-p activation... 

P2Y6 Some epithelial cells, placenta, T cells, thymus UDP > UTP > > ATP, UDP 3S, IDP MRS2578 Gq/Gn PLC-p activation... 

The for the substrate UTP has been measured and does not show significant differences between wt and mutant enzymes. The model shows that the space available to substituents in positions 4 and 5 of the thiophene is limited, in agreement with SAR studies. Interaction with a number of basic and lipophilic residues bound... 

Glucose-6-phosphate interacts with UTP to form UDPD-glucose and pyrophosphate. 

By means of the enzyme nucleoside diphosphate kinase, UTP, GTP, and CTP can be synthesized from their diphosphates, eg,... 

In animal tissues the synthesis of UDPGlc from UTP and glucose-1-phosphate is catalysed by a pyrophosphorylase of widespread occurrence. With the enzyme from human liver, there is no specificity for either the... 

UTP Uridine triphosphate UV Ultraviolet UVA Ultraviolet A UVB Ultraviolet B UVR Ultraviolet irradiation UW University of Wisconsin (preserving solution)... 

While metal-nitrogen and metal-oxygen bonded compounds dominate nucleobase coordination chemistry, examples in which metal-carbon bonds are formed have been identified. Early studies on the synthesis of metal-labeled DNA demonstrated that nucleotide-triphosphates, UTP, CTP, dUTP, and dCTP, can undergo mercury modification at C5 (82,83). The UTP derivative was also shown to act as a substrate for RNA polymerase in the presence of mercaptans (83). Later, guano-sine was shown to undergo mercury modification at C8 though, in this case, the purine was multiply substituted, 21 (84). 

Figure 12 Gradient separation of bases, nucleosides and nucleoside mono- and polyphosphates. Column 0.6 x 45 cm. Aminex A-14 (20 3 p) in the chloride form. Eluent 0.1 M 2-methyl-2-amino-l-propanol delivered in a gradient from pH 9.9-100 mM NaCl to pH 10.0-400 mM NaCl. Flow rate 100 ml/hr. Temperature 55°C. Detection UV at 254 nm. Abbreviations (Cyt) cytosine, (Cyd) cytidine, (Ado) adenosine, (Urd) uridine, (Thyd) thymidine, (Ura) uracil, (CMP) cytidine monophosphate, (Gua) guanine, (Guo) guanosine, (Xan) xanthine, (Hyp) hypoxanthine, (Ino) inosine, (Ade) adenosine, (UMP) uridine monophosphate, (CDP) cytidine diphosphate, (AMP) adenosine monophosphate, (GMP) guanosine monophosphate, (IMP) inosine monophosphate, (CTP) cytidine triphosphate, (ADP) adenosine diphosphate, (UDP) uridine monophosphate, (GDP) guanosine diphosphate, (UTP) uridine triphosphate, (ATP) adenosine triphosphate, (GTP), guanosine triphosphate. (Reproduced with permission of Elsevier Science from Floridi, A., Palmerini, C. A., and Fini, C., /. Chromatogr., 138, 203, 1977.)...

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