Uridine diphosphate glucose preparation

F5. Fevery, J., Leroy, P., Van De Vijver, M., and Heirwegh, K. P. M., Structures of bilirubin conjugates synthesized in vitro from bilirubin and uridine diphosphate glucuronic acid, uridine diphosphate glucose or uridine diphosphate xylose by preparations from rat liver. Biochem. J. 129, 635-644 (1972). 

Phosphorolysis of ribonucleic acid with polynucleotide phosphorylase gives a mixture of the diphosphates of the four common nucleosides, which are transformed into triphosphates with enolpyruvate phosphate and pyruvate kinase. This mixture may be used as such as a source of uridine triphosphate in the preparation of the nucleotide-sugar uridine 5 -(a-D-glucopy-ranosyl diphosphate) ( uridine-diphosphate-glucose, UDP-Glc), or as a... 

These compounds are glycosyl esters of nucleoside mono- or diphosphates. A number have been found in cells, but only two have so far been considered in the present context. The preparation of uridine diphosphate glucose" (48) is possible independent of its further transformation (see Table... 

Stoichiometric quantities of uridine diphosphate glucose were used, in the presence of a transfer enzyme, sucrose synthetase, in the soluble state (extraction given). Coupling with modified D-fructose gave sucroses modified on the D-fructosyl group, on the 1 -3-mmol scale. Thus were prepared l -deoxy-l -fluoro- (59%),98 4 -deoxy-4 -fluoro- (16%), and l -azido-l -deoxy-sucrose (15%).71 6-Deoxy-6-fluoro-D-glucose was isomerized to 6-deoxy-6-fluoro-D-fructose with isomerase, and gave 6 -deoxy-6 -fluoro-sucrose.71... 

D-Glucose 2-phosphate is formed as decomposition product of uridine diphosphate glucose with cyclic 1,2-glucose phosphate as intermediate . It has been prepared by phosphorylation of r,3,4,6-tetraacetyl-/ D-glucose with diphenyl phosphorochloridate . 

The intravenous injection of 2-amino-2-deoxy-D-glucose-14C into rats, and subsequent isolation of the uridine diphosphate N-acetyl-hexosamine fraction, has been suggested as a fast and convenient method for preparing small amounts of a mixture of radioactive uridine 5 -(2-acetamido-2-deoxy-a-D-gIucopyranosyl pyrophosphate) and uridine 5 -(2-acetamido-2-deoxy-a-D-galactopyranosyl pyrophosphate). 

Flowers, H.M., Batra, K.K., Kemp, J. Hassid, W.Z. (1968) Biosynthesis ot insoluble Glucans from Uridine Diphosphate-D-glucose with Enzyme Preparations from Phaseolus aureus and Lupinus albi, Plant Physiology, 43, 1703-9 Forsee, W.T., Griffin, J.A. Schutzbach, J.S. (1977) Mannosyltransfer fi om GDP-Mannose to Oligosaccharide Lipids , Biochemical and Biophysical Research Communications, 75, 799-805... 

Synthesis of sucrose 6 -phosphate by an enzymic method using uridine 5 -(a-D-glucopyranosyl diphosphate) plus D-glucose 6-phosphate has been reported.126-131 The first, unambiguous, chemical synthesis of sucrose 6 -phosphate was achieved by Buchanan and coworkers.18 The reaction of 2,3,4,6,l, 3, 4 -hepta-0-acetylsucrose, prepared by five steps of synthesis, with cyanoethyl phosphate in pyridine gave a crude product from which sucrose 6 -phosphate was isolated as the barium salt. 

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