Urease test

Drugs were given for 2 weeks and eradication checked 1 month after stopping treatment with rapid urease test and histology on biopsy samples taken from the antrum and the corpus. ... 

Phenotypical isolates from purified water shall be characterized. Biochemical testing (such as oxidase test, urease test, catalase test, citrate test, coagulase test, and indole test) and commercial test kits (such as API tests) and reagents may be used for conformation of some unique isolates. 

All species of Cryptococcus are nonfermentative aerobes exhibiting a positive urease test, a positive diazonium blue B test, and the presence of extracellular amyloid compounds. Cryptococcus differs from Trichosporon by the presence of capsules and the absence of arthroconidia. Genotypic methods are necessary to identify the species unequivocally [208]. 

H. pylori produces urease, and hydrolysis of endogenous urea to bicarbonate and ammonia may create a more hospitable microenvironment for the survival of the organism in the stomach. Mammalian cells do not hydrolyze urea and it was only in 1984 that gastric urease was associated with the presence of H. pylori The ability of the organism to rapidly hydrolyze urea is the basis of the urea breath tests and of the direct urease tests on gastric biopsy samples. 

Histology microscopy after Giemsa or silver staining Histology microscopy after immunohistochemical staining Direct urease test a biopsy incubated in urea/indicator solution visual endpoint... 

All resulting colonies were evaluated to verily their Brucella origin via oxidase, catalase, and urease tests. All colonies were confirmed to be Brucella and grew on the appropriate antibiotic-supplemented media. 

Test your urease test strips as follows. Tear off about a 1 cm piece of the urease-bromthymol blue test paper and place it on a microscope slide. Pipet one drop of urine onto the test paper, and cover it with a second microscope slide, or cover plate. If urea is present, the paper will turn blue in a few minutes. 

When combined with proton pump inhibitor (PPI) and other antimicrobials for the therapy of duodenal ulcer and chronic active gastritis, roxithromycin showed excellent activity in eradicating H. pylori. Eradication rates of 80-100% as determined by urease test, histologic analysis, and microbiologic cultures were achieved in various randomized open clinical trials [174-176]. However, lower rates of cure (50-67%) were found in larger randomized comparative studies [177,178]. 

Frequently swarms, IMViC tests, rapid positive urease test. 

Under these circumstances, a biopsy sample should be used to test for H. pylori, preferably by means of the rapid urease test. It is important that at least two biopsy samples be evaluated (fundic and antral), because if eradication therapy has not been completely successful, the organism may be present only in small numbers and may have migrated (a consequence of acid-suppressive therapy and the alteration of its ideal pH milieu) from its original site. In those patients who exhibit relapse or recurrence of symptomatology, the presence of H. pylori should be meticulously assessed. 

Rapid urease test Breath test Biopsy Culture Serology... 

Urease test (specific for urea). Add a few drops of phenolphthalein to a solution of 0 - 2 g of the substance in 5 ml of water, followed by a pinch of jack bean meal or a crushed urease tablet. Shake the mixture, which slowly becomes pink due to the hydrolysis of urea to ammonium carbonate (alkaline). 

It is important to detect H. pylori infection because of its association with nonjunctional gastric adenocarcinoma, gastritis and duodenal ulceration, B-cell lymphoma, and reflux oesophagitis. There are a number of mechanisms for its detection. The urea breath test is the most accurate noninvasive test with an accuracy of 96% (Cohne et al. 1999). The rapid urease test or CLO test is performed at the time of endoscopy and has a 97% accuracy and an 80% sensitivity. This can indicate infection within minutes. Combining the CLO test with microscopy of tissue specimens, the detection rate comes close to 100%, emphasizing the importance of several biopsies from the gastric antrum (GuR et al. 1998). 

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