Ultraviolet detection comparisons

P Hayes, MR Smyth, I McMurrough. Comparison of electrochemical and ultraviolet detection methods in high-performance liquid chromatography for the determination of phenolic compounds commonly found in beers. Part 1. Optimization of operating parameters. Analyst 112 1197-1203, 1987. 

Awidi A et al (2010) Determination of imatinib plasma levels in patients with chronic myeloid leukemia by high performance liquid chromatography-ultraviolet detection and liquid chromatography-tandem mass spectrometry methods comparison. Leuk Res 34 714-717... 

L4. Lykkesfeld, J., Determination of malondialdehyde as dithiobarbituric acid adduct in biological samples by HPLC with fluorescence detection Comparison with ultraviolet-visible spectrophotometry. Clin. Chem. 47, 1725-1727 (2001). 

Lykkesfeld, J. (2001). Determination of Malonaldehyde as Dithiobarbituric Acid Adduct in Biological Samples by HPLC with Fluorescence Detection Comparison with Ultraviolet-Visible Spectrophotometry, Clinical Chemistry, Vol.47, pp.l725-... 

Clark GJ, Goodin RR, Smiley JW. 1985. Comparison of ultraviolet and reductive amperometric detection for determination of ethyl and methyl parathion in green vegetables and surface water using high-performance liquid chromatography. Anal Chem 57 2223-2228. 

The principal limitation in the use of electrophoretic techniques is the lack of availability of suitable detection systems for quantitative analysis and unequivocal identification of pesticide analytes. Traditionally, either ultraviolet/visible (UVA IS) or fluorescence detection techniques have been used. However, as with chromatographic techniques, MS should be the detection system of choice. A brief comparison of the numbers of recent papers on the application of GC/MS and LC/MS with capillary elec-trophoresis/mass spectrometery (CE/MS) demonstrates that interfaces between CE... 

FBAs can also be estimated quantitatively by fluorescence spectroscopy, which is much more sensitive than the ultraviolet method but tends to be prone to error and is less convenient to use. Small quantities of impurities may lead to serious distortions of both emission and excitation spectra. Indeed, a comparison of ultraviolet absorption and fluorescence excitation spectra can yield useful information on the purity of an FBA. Different samples of an analytically pure FBA will show identical absorption and excitation spectra. Nevertheless, an on-line fluorescence spectroscopic method of analysis has been developed for the quantitative estimation of FBAs and other fluorescent additives present on a textile substrate. The procedure was demonstrated by measuring the fluorescence intensity at various excitation wavelengths of moving nylon woven fabrics treated with various concentrations of an FBA and an anionic sizing agent. It is possible to detect remarkably small differences in concentrations of the absorbed materials present [67]. 

Anderson, M. S., Lu, B., Abdel-Rehim, M., Blomberg, S., and Blomberg, L. G. (2004). Utility of nonaqueous capillary electrophoresis for the determination of lidocaine and its metabolites in human plasma a comparison of ultraviolet and mass spectrometric detection. Rapid Commun. Mass Spectrom. 18, 2612—2618. 

The sensitivity of the Gutzeit test varies with the conditions. Variation in the humidity of the gas should be avoided.8 Under ordinary laboratory conditions 10-6 g. of As may be detected, but amounts of 1, 2 and 3 x 10-6 g. are not easily differentiated.9 If the quantity of arsenic in the aliquot test portion is as much as 0-04 mg. the comparison of the stains is not satisfactory.10 It has been observed that arsenic stains which are invisible to the naked eye become strikingly visible under ultraviolet rays.11... 

Tor reference. Positive identification can be made only by collecting the compound or transierring it as it elutes directly into another apparatus for analysis by other means, such as infrared or ultraviolet spectroscopy, mass spectrometry, or nuclear magnetic resonance. Commercially available apparatus is available which combines in a single unit both a gas chromatograph and an infrared, ultraviolet, or mass spectrometer for routine separation and identilicalion. The ancillary system may also be microprocessor-based, with an extensive memory for storing libraries of known infrared spectra or fragmentation patterns (in the case of mass spectrometers). Such systems allow microprocessor-controlled comparison and identilicalion of detected compounds. 

Carando S, Teissedre PL and Cabanis JC, Comparison of (+)-catechin determination in human plasma by high-performance liquid chromatography with two types of detection Fluorescence and ultraviolet. J Chromatogr B 707 195-201 (1998). 

Atienzar FA, Cordi B, Donkin ME, Evenden AJ, Jha AN, Depledge MH. 2000. Comparison of ultraviolet-induced genotoxicity detected by random amplified polymorphic DNA with chlorophyll fluorescence and growth in a marine macroalgae, Palmaria palmata. Aquat Toxicol 50 1-12. 

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