Fluorescent detection technique

Fluorescence. The fluorescence detection technique is often used in clinical chemistry analyzers for analyte concentrations that are too low for the simpler absorbance method to be appHed. Fluorescence measurements can be categorized into steady-state and dynamic techniques. Included in the former are the conventional simultaneous excitation-emission method and fluorescence polarization. 

The principal limitation in the use of electrophoretic techniques is the lack of availability of suitable detection systems for quantitative analysis and unequivocal identification of pesticide analytes. Traditionally, either ultraviolet/visible (UVA IS) or fluorescence detection techniques have been used. However, as with chromatographic techniques, MS should be the detection system of choice. A brief comparison of the numbers of recent papers on the application of GC/MS and LC/MS with capillary elec-trophoresis/mass spectrometery (CE/MS) demonstrates that interfaces between CE... 

The IR spectra recorded by the fluorescence detection technique, where the population of a species in the ground state is monitored by laser-induced fluorescence with UV laser excitation, can be used successfully to detect dihydrogen bonds in the gas phase. It has been found, for example, that the v(O-H) and v(N-H) stretching vibrations in such IR experiments are very sensitive to dihydrogen bonding. 

A host of detection principles can be used to sense the presence of double-stranded DNA. Fluorescent detection techniques employ dyes that change fluorescence properties upon binding to DNA (this is also discussed in detail in Chapter 2.5). 

Flow cytometry is a high-precision technique for rapid analysis and sorting of cells and particles. The principles underlying flow cytometry are discussed in many comprehensive review articles (3-5) and will only be touched on here. This fluorescent detection technique provides statistical accuracy, reproducibility, and sensitivity, as well as allowing the simultaneous measurement of multiple parameters on a cell-to-cell basis. Information is derived from the optical responses of... 

On the other hand, spectroscopic detection methods, such as laser-induced fluorescence, UV/Vis absorption, chemiluminescence, and thermal lens microscopy, have been used for on-chip detection. Among these methods, the fluorescence detection method has been most widely used because of its high sensitivity and low detection limits for biologically relevant species. However, the fluorescence detection technique has some disadvantages. Many chemical... 

During the last 15 years there has been a renaissance of fluorescence, mainly driven by (1) recent advances in fluorescence detection techniques (2) the availability of powerful data acquisition and data analysis systems (3) the development of an array of improved fluorescent dyes and labels and (4) the increasing... 

Resonance scattering (also known as atomic or molecular resonance fluorescence) exhibits relatively large cross-sections however, quenching of the fluorescence by the more abundant ground-state species at atmospheric pressure is responsible for low detected signals. Hence, this technique is more useful in low-pressure environments, e.g. to detect trace components in the upper atmosphere. When using absorption or fluorescence detection techniques the... 

---