Column TSK gel

SIZE EXCLUSION CHROMATOGRAPHY USING TSK-GEL COLUMNS AND TOYOPEARL RESINS... 

TSK-GEL column Particle size (/tm) Average pore size (A) Polyethylene oxides/glycols Molecular weight of sample Dextrans Globular proteins ... 

There are several basic guidelines to follow when cleaning a column. These rules apply regardless of the type of TSK-GEL column that is being cleaned. 

Dehydration of TSK-GEL columns can result from improper use or during long-term storage. This condition can be remedied by the following procedure. 

The TSK-GEL column is a trademark of Tosoh Haas Co. Ltd and the Waters Insulin HMWP column is a trademark of Waters Corporation. The author thanks the book s editor Chi-san Wu, the assigned peer reviewer, and the Eli Lilly and Company reviwers John Towns, Ralph Riggin, and James Kelley for their assistance, time, and professional comments. 

Chromatography System A Hewlett-Packard 1050 Liquid Chromatography System (HP 1050) with a Quaternary Pump was used for separations. The separations were monitored with a Hewlett-Packard 1050 Variable Wavelength Detector (280 nm) and an autosampler was used to introduce samples into the column. For this application a TSK-Gel column (SP-5PW 07161, TosoHaas, Stuggart, Germany) with dimensions 7.5 cm X 7.5 mm was used. Data were acquired with the Hewlett-Packard ChemStation software which controls the HP1050 Liquid Chromatograph. 

A few precautionary measures will ensure the efficient operation of TSK gel columns ... 

Fig. 3. Elution patterns of the total lipoprotein fraction by a single TSK GEL column. Column (a), G3000SW (b), G4000SW (c), G5000PW (d), G6000PW. Eluent 0.1 M Tris-HCl buffer (pH 7.4). Flow rate 1.0 ml/min. Loaded volume 20 pi. Peaks 1, chylomicrons 2, VLDL 3, LOL 4, HDL 5, albumin.

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