Trichoderma viride cellulase, hydrolysis

Howell, J. A. and J. D. Stuck, "Kinetics of Solka Floe Cellulose Hydrolysis by Trichoderma viride Cellulase," Biotechnol. Bioeng. 17 (1975) 873-893. 

Table VIII. Hydrolysis of Cellulose Materials by Trichoderma viride Cellulase ...

The three cellulases decomposed about 25-45% of the cellulose accompanied by solubilization of about 40-70% of the mannan and, by partial hydrolysis, of about 20% of the xylan present in the untreated sprucewood holocellulose. Based on the degradation products (cf. Table III, Columns 13-15, and Table II), the catalytic actions of the three cellulases—all isolated from Trichoderma viride—are similar or identical. The lower absolute degradation values obtained with cellobiohydrolase C might merely be a result of enzyme concentration. 

Gong C-S, Ladisch MR, Tsao GT (1977) Cellobiase from Trichoderma viride-. purification, properties,kinetics, and mechanism. Biotechnology and Bioengineering 19 959-981 Gong C-S, Ladisch MR, Tsao GT (1979) Biosynthesis, purification and mode of action of cellulase of Trichoderma reesei-. hydrolysis of cellulose mechanisms of enzymatic and acid catalysis. Advances in Chemistry Series vol. 181, American Chemical Society, Washington, DC, pp 261-287... 

Mandels et al. (22) found that glucose manufactured commercially by acid hydrolysis of starch contained sophorose as an impurity which is a powerful inducer of cellulase in Trichoderma viride. In the studies... 

A number of commercial cellulases, samples from other workers, and acetone precipitated powders were compared (Table II). All of these demonstrated good activity on carboxymethylcellulose. In fact a number of these, notably the enzyme from Poria, were more active on CMC than the T. viride enzyme. About half of the enzymes showed good activity on filter paper. But T. viride enzymes show the highest activity on cotton, the most resistant substrate. In case inactivation at 50 °C. affected the activity on cotton and filter paper, these enzymes were also tested for hydrolysis at 25°C. (data not shown). The relative activities were the same as those found at 50°C. Selby (31) made a similar study of a number of cellulase preparations. He also found that the cellulase of Trichoderma viride had the highest activity on cotton. 

Evidence for Ex-1 to be an Exo-type Component. The time course of CMC hydrolysis by Ex-1 is shown in Figure 10. The hydrolysis proceeded rapidly at first, but it reached a plateau and seemed to stop after 3 hr. This is characteristic of the hydrolysis by exo-type cellulase, as has been reported for exocellulase of glucosidase type from T. viride (7) and for another Trichoderma exocellulase of Avicelase type (10). 

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