Trichoderma reesei, hydrolysis

Gong C-S, Ladisch MR, Tsao GT (1977) Cellobiase from Trichoderma viride-. purification, properties,kinetics, and mechanism. Biotechnology and Bioengineering 19 959-981 Gong C-S, Ladisch MR, Tsao GT (1979) Biosynthesis, purification and mode of action of cellulase of Trichoderma reesei-. hydrolysis of cellulose mechanisms of enzymatic and acid catalysis. Advances in Chemistry Series vol. 181, American Chemical Society, Washington, DC, pp 261-287... 

Table VIL Comparison of Hydrolysis of Delignified Wheat Straw with Cellulase Systems of Trichoderma reesei QMY-1 Produced Under Solid-State Fermentation ...

The xylanolytic enzyme system of Trichoderma reesei, a well-known producer of cellulolytic enzymes, is versatile and well suited for the total hydrolysis of different xylans. It consists of two major, specific and several non-specific xylanases, at least one / -xylosidase, a-arabinosidase and a-glucuronidase and at least two acetyl esterases. The hydrolysis of polymeric xylans starts by the action of endoxylanases. The side-groupcleaving enzymes have their highest activities towards soluble, short xylo-oligosaccharides, and make the substituted oligosaccharides again accessible for xylanases and / -xylosidase. 

While Trichoderma reesei is best known as an efficient producer of cellulolytic enzymes, it has also been reported to produce xylanase and / -xylosidase (18-20). Two xylanases and a / -xylosidase have been purified from T. reesei (10), and two xylanases (21,22) and a / -xylosidase (5) from T. viride. We have previously shown that T. reesei produces all the enzymes needed for complete hydrolysis of native substituted xylans (23). One xylanase (24), a / -xylosidase (25), an a-arabinosidase (26), and an acetyl esterase (27) of T. reesei have so far been purified. In this chapter, the mode of action of these enzymes in the hydrolysis of different xylans is discussed. 

Index Entries Trichoderma reesei fermentation cellulase growth characterization cellulose hydrolysis. 

Cavaco-Paulo, A., Almeida, L., and Bishop, D. 1998. Hydrolysis of Cotton Cellulose by Engineered Cellulases from Trichoderma Reesei. Textile Res. J., 68(4), 273-280. 

Medve, J., Karlsson, J., Lee, D., and Tjerneld, F. 1998. Hydrolysis of microcrystalline cellulose by cellobiohydrolase I and endoglucanase II from Trichoderma reesei Adsorption, sugar production pattern and synergism of the enzymes. Biotech. Bioeng.,59, 621-634. 

Nidetzky, B., Steiner, W, Hayn, M., and Claeyssens, M., Cellulose hydrolysis by the cellulases from Trichoderma reesei A new model for synergistic interaction. Biochem J 1994, 298 Pt 3, 705-10. 

Poutanen, K. and Sundberg, M., An acetyl esterase of Trichoderma reesei and its role in the hydrolysis of acetyl xylans. Appl. Microbiol. Biotechnol. 1988, 28, 419-424. 

Other Uses. Rapeseed or canola meal has been used as a fermentation substrate. It has been included as an additive in compost for commercial button mushroom Agaricus bisporus) production with good success (32). Canola meal has also been tested as a substrate for xylanase production by Trichoderma reesei. Results from this work indicate that the hydrolysis of canola meal by this enzyme system might be useful in converting this material to fermentable sugars that could be further processed to expensive end products such as solvents and chemicals (33). 

Ladisch MR, Gong C-S, Tsao GT (1980) Cellobiose hydrolysis by endoglucanase (glucan glucanohydrolase) from Trichoderma reesei-. kinetics and mechanism. Biotechnology and Bioengineering 22 1107-1126... 

Ladisch MR, Lin KW, Voloch M, Tsao GT (1983) Process considerations in the enzymatic hydrolysis of biomass. Enzyme and Microbial Technology 5 82-102 Kleywegt GJ, Zou J-Y, Divine, Dales C, Gideon J, Sinning 1, Stahlberg J, Reinikainen T, Srisodsuk M, Terri TT, Jones TA (1997) The crystal structure of the catalytic core domain of endoglucanase I from Trichoderma reesei at 3.6 A resolution, and a comparison with related enzymes. Journal of Molecular Biology 272 383-397... 

Medve J, Stahlberg J, Tjernel F (1994) Adsorption and synergism of cellobiohydrolase I and II from Trichoderma reesei during hydrolysis of microcrystalline cellulose. Biotechnology and Bioengineering 44 1064 -1073... 

Discrete CBMs are usually produced by protein engineering methods however, with some enzymes, such as the cellulase components of Trichoderma reesei, the CBMs are connected to the catalytic by long, heavily glycosylated linker regions, which are susceptible to attack by proteases, so that protease hydrolysis of commercial cellulases can be practical method of obtaining CBMs. ... 

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