Protease hydrolysis

Protease is used in detergents to remove protein-based stains such as blood, mucus, feces, and various foods such as egg and gravy. These substances are almost insoluble and they tend to adhere to textiles and other surfaces. The protease hydrolysis products are peptides which are readily dissolved or dispersed in the washing liquor. 

Protease Hydrolysis of proteins and polypeptides. Lipase Hydrolysis of triglycerides of simple fatty acids. 

Protease Hydrolysis of amide linkages in proteins and peptides... 

Hydrolases Lipases Nucleases Proteases Hydrolysis of an ester group Hydrolysis of a phosphate group Hydrolysis of an amide group... 

The answer is d. (Murray, pp 48-62. Scriver, pp 3 5. Sack, pp 1-3. Wilson, pp 101-120.) Pepsin is secreted in a proenzyme form in the stomach. Unlike the majority of proenzymes, it is not activated by protease hydrolysis. Instead, spontaneous acid hydrolysis at pH 2 or lower converts pepsinogen to pepsin. Hydrochloric acid secreted by the stomach lining creates the acid environment. All the enzymes secreted by the pancreas are activated at the same time upon entrance into the duodenum. This is accomplished by trypsin hydrolysis of the inactive proenzymes trypsino-gen, chymotrypsinogen, procarboxypeptidase, and proelastase. Primer... 

Discrete CBMs are usually produced by protein engineering methods however, with some enzymes, such as the cellulase components of Trichoderma reesei, the CBMs are connected to the catalytic by long, heavily glycosylated linker regions, which are susceptible to attack by proteases, so that protease hydrolysis of commercial cellulases can be practical method of obtaining CBMs. ... 

Jung, S. RA. Murphy L.A. Johnson. Physicochemical and functional properties of soy protein substrates modified by low levels of protease hydrolysis./. Food Sci. 2005, 70, C180-187. 

Proteases Hydrolysis of proteins Proteases immobilized in albu-min/chitosan gel [22]... 

The peptidic produets fixnn the V8-protease hydrolysis of bovine hemoglobin were studied on a C4 column (A = 226 nm) using a 35-min 100/0 -> 33/67 water (0.1% TFA)/(40/60 water [0.1% TFA]/acetonitrile) gradient [1290]. Pbptide components from the hydrolysates of 2% w/v hemoglobin samples were readily detected. 

It is reasonable to inquire whether recognition of such a concept might apply to mechanistic ideas about enzyme reactions. It now appears that the stereoelectronic theory that Deslongchamps and co-workers have developed is specifically applicable to peptide hydrolyses by serine proteases. Hydrolysis of simple esters will be used first to illustrate the approach. 

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