Trichloroethylene, degradation Burkholderia cepacia

Domingo et al. evaluated immobilization of trichloroethylene-degrading Burkholderia cepacia bacteria using hydrophilic polyurethane foam. The influences of several foam formulation parameters upon cell retention were examined. Surfactant type was a major determinant of retention a lecithin-based compound retained more cells than Pluronic- or silicone-based surfactants. 

Mars, A.E., J. Houwing, J. Dfolfing, and D.B. Janssen. 1996 Degradation of toluene and trichloroethylene by Burkholderia cepacia G4 in growth-limited fed-batch culture. Appl. Environ. Microbiol. 62 886-891. 

Munakata-Marr, J., P.L. Mccarty, M.S. Shields, M. Reagin, and S.C. Francesconi. 1996. Enhancement of trichloroethylene degradation in aquifer microcosms bioaugmented with wild-type and genetically altered Burkholderia Pseudomonas cepacia G4 and PR1. Environ. Sci. Technol. 30 2045-2052. 

This chapter describes an evanescent wave fiber optic biosensor and its application to immunoassays for rapid detection of bacterial cells and pollutants. Whole cells of Burkholderia cepacia G4 5223-PRl (G4) are of interest for their ability to degrade trichloroethylene (TCE) which is one of the most prevalent contaminants of ground water in the United States. The lower limit of detection of the G4 with this system is 10 - 10 cells/ml. In addition to TCE, the explosive trinitrotoluene (TNT) is a known contaminant of ground water. Limits of detection of TNT with this system is 10 ng/ml. 

Mars, A.E, Houwing, I, Dolfing, J. Janssen, D.B. 1996. Fed-Batch Culture by Burkholderia cepacia G4 in growth-Umited degradation of toluene and trichloroethylene. Appl Environ. Microbiol. 62(3) 886. 

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