Tricaine methanesulfonate

McKim, J.M., P.K. Schmieder, R.W. Carlson, and E.P. Hunt. 1987. Use of respiratory-cardiovascular responses of rainbow trout (Salmo gairdneri) in identifying acute toxicity syndromes in fish. 1. Pentachlorophenol, 2,4-dinitrophenol, tricaine methanesulfonate and 1-octanol. Environ. Toxicol. Chem. 6 295-312. 

Tributyltins, 24 817 as skin irritants, 24 829 Tricadmium diarsenide, 4 510 Tricadmium diphosphide, 4 510 Tricaine methanesulfonate... 

Tricaine methanesulfonate Used in fish as a chorionic gonadotropin to enhance spawning. Apphed intramuscularly, i.e., as an injectable. 

Tricaine methanesulfonate (MS-222). Tricaine methanesulfonate (3-aminobenzoic acid ethyl ester methanesulfonate MS-222) is widely used for the sedation and anesthetization of fish. The compound is 0.01% ionized at body pH and has a partition coefficient of 312. This relatively nonpolar lipophilic drug is rapidly absorbed from water and eliminated in freshwater and marine fish species. 

Figure 4. Biotransformation cascade of Tricaine methanesulfonate (MS-222) in fish. A,B., Tricaine methanesulfonate equilibrium C. Ethyl-3-acetylaminobenzoate D., 3-acetylaminobenzoic acid E., 3-aminobenzoic acid.

Tricaine methanesulfonate (MS222, aminobenzoic acid ethyl ester. Sigma A-5040), 0.1% sodium bicarbonate. Dissolve in water. 

Zebrafish anesthesia Prepare a stock solution of 400 mg of tricaine (ethyl 3-aminobenzoate methanesulfonate) in 97.5 mL of egg water. 

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