TRAP total radical-trapping antioxidant

TRAP Total radical-trapping antioxidant Rice-Evans447... 

Other measurements of antioxidant activity include FRAP (ferric reducing-antioxidant power) (123-126), TRAP (total radical-trapping antioxidant parameter) (123, 127), phycoerythrin assay (128, 129), and test of metal chelating capacity (130, 131), among others. Reviews on methods for testing antioxidant activity have been published (9, 12). 

Com oil phenols have been studied with respect to their potential to scavenge the free radicals, the peroxyl radicals, the superoxide radicals, etc. The free radical scavenging activity of the phenolic acids using model free radicals such as l,7-diphenyl-2-picrylhydrazyl radical (DPPH ), or ABTS (2,2 -azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt cation radical) is often measured. TEAC (Trolox equivalent antioxidant capacity), FRAP (Ferric-reducing antioxidant capacity) and TRAP (total-radical-trapping antioxidant parameter) of the com oil are also used in order to obtain additional information, necessary to investigate the relation between the antioxidant intake and the oxidative stress related diseases (Stratil et al., 2008 Ceto et al., 2014 Apak et al., 2007). 

Wayner, D., Burton, G., Ingold, K.U. and Locke, S. (1985). Quantitative measurement of total peroxyl radical trapping antioxidant capability of human blood plasma by controlled peroxidation. FEBS Lett. 187, 33-37. 

Wayner, D.D., Burton, G.W., Ingold, K.U., Barclay, L.R.C. and Locke, S.J. (1987). Antioxidants in human blood plasma. The relative contributions of vitamin E, urate, ascorbate and protein to the total radical trapping antioxidant activity. Biochim. Biophys. Acta 925, 408-413. 

TRAP Assay (Total Radical-Trapping Antioxidant Parameter)... 

DCFH-DA assay was first developed by Valkonen and Kuusi (1997) as an alternative to measure the total peroxyl radical-trapping antioxidant potential of plasma. This assay uses AAPH to generate peroxyl radicals and DCFH-DA as the oxidizable substrate for the generated radicals. The oxidation of DCFH-DA by peroxyl radicals converts DCFH-DA to dichlorofluorescein. DCF is highly fluorescent (excitation 480 nm, emission 526 nm) and also shows absorbance at 504 nm. Therefore, the produced DCF can be monitored either fluorometrically or spectrophotometrically. 

Valkonen M and Kuusi T. 1997. Spectrophotometric assay for total peroxyl radical-trapping antioxidant potential in human serum. J Lipid Res 38(4) 823-833. 

Wayner DD, Burton GW, Ingold KU Barclay LR Locke SJ. 1987. The relative contributions to the total peroxyl radical-trapping antioxidant activity of human blood plasma. Biochim Biophys Acta 924 408-419. 

Total radical trapping parameter (TRAP) assay is widely used in investigations and has various modifications [45-48]. This method presumes antioxidants capability to react with peroxyl radical 2.2-azobis (2-amidinopropane) dihydrochloride (AAPH). TRAP modifications differ in methods of registering analytical signal. Most often the final stage of analysis include peroxyl radical AAPH reaction with luminescent (luminol), fluorescent (dichlorofluorescein-diace-tate, DCFH-DA) or other optically active substrate. Trolox is often used as a standard. 

TRAP, Total peroxyl-radical-trapping antioxidant capability of plasma TEAC, Trolox equivalent antioxidant capacity FRAP, ferric-reducing activity of plasma ORAC, oxygen-radical-absorbing capacity PCA, perchloric acid-soluble fraction ABAP, 2,2 -azobis(2-amidoproane). 

TRAP, Total peroxyl-radical-trapping antioxidant capability of plasma. 

K4. Kharb, S., Total free radical trapping antioxidant potential in pre-eclampsia. Int. J. Gynaecol. Obstet. 69,23-26 (2000). 

TRAP Total peroxyl radical scavenging antioxidant capacity 3 (113)... 

Total radical-trapping antioxidant parameter pmol peroxy radical deactivated... 

More recently, studies on the absorption of flavonoid glycosides typical of berries and grapes are reported. Lapidot et al. (1998) traced anthocyanins in human urine after the intake of 300 ml red wine, corresponding to 218 mg of anthocyanins. Totals of 1.5-5.1% of the anthocyanins were recovered in the urine within 12 h after wine consumption, two compounds were unchanged, whereas other compounds seemed to have undergone molecular modifications. The anthocyanin levels of the urine reached a peak within 6 h of consumption. Serafini et al. (1998) tested the effects of the intake of the nonalcoholic fraction of red or white wine on plasma antioxidant capacity, measured as total radical-trapping parameter (TRAP), and on... 

TRAP (total radical antioxidant parameter) assay 857... 

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