Transfection with mesenchymal stem

Yoshikawa T, Uchimura E, Kishi M, Funeriu DP, Miyake M, Miyake J (2004) Transfection microarray of human mesenchymal stem cells and on-chip siRNA gene knockdown. J Control Release 96 227-232 Zhang Y, Feng XH, Derynck R (1998) Smad3 and Smad4 cooporale with c-Jun/c-Fos to mediate TGF-beta-induced transcription. Nature 394 909—913... 

Zhang, Y., Zhang, H., Zhang, G., Ka, K., Huang, W, 2014. Combining acellular nerve allografts with brain-derived neurotrophic factor transfected bone marrow mesenchymal stem cells restores sciatic nerve injury better than either intervention alone. Neural Regen. Res. 9, 1814-1819. 

Mesenchymal stem cells harvested from rabbits were induced into osteoblasts in vitro and were cultured for a week, statically loaded onto the porous hydroxyapatite + chitin matrices and implanted into bone defects of the rabbit femur for 2 months. The histology of explants showed bone regeneration with the biodegradation of the hydroxyapatite + chitin matrix. Similarly, green fluorescence protein transfected mesenchymal stem cells-induced osteoblasts were also loaded onto porous hydroxyapatite + chitin matrices and implanted into the rabbit femur. Mesenchymal stem cells-induced osteoblasts did not only proliferate but also recruited surrounding tissue to grow in. The hydroxyapatite + chitin matrix qualified for tissue-engineered bone substitutes (Ge et al. 2004). 

Park JS, Na K, Woo DG et al (2010) Non-viral gene delivery of DNA polyplexed with nanoparticles transfected into human mesenchymal stem cells. Biomaterials 31 124—132... 

Yang, J. R, Zhou, W. W., Zheng, W., Ma, Y. L., Lin. L.. Tang. T.. Liu. J. X.. Yu. J. R. Zhou. X. M.. and Hu. J. G. 2007. Effects of myocardial transplantation of marrow mesenchymal stem cells transfected with vascular endothelial growth factor for the improvement of heart function and angiogenesis after myocardial infarction. Cardiology, 107,17-29. 

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