Transcription splicing

Gene recognition involves the identification of DNA functional elements as well as signals recognized by the transcriptional, splicing and translational machinery. The... 

Transcriptional Splicing Eupoil/lmport Lifetime Translational Lifetime... 

Members of the Parvovirus genus cause a number of diseases in mammals, including enteritis [37, 38] and childhood fifth disease. These nonenveloped viruses have a capsid with a diameter of 255 A that encases a double-stranded DNA genome and infect only cells that are in the S phase [39]. In canine parvovirus (GPV) the T=1 capsid is composed mainly of 60 copies of viral protein 2 (VP2). There is a small amount of VPS in the capsid that is the result of proteolytic cleavage of 17 residues from the amino terminus of VP2. Up to 20% of the 60 copies of VP2 in each capsid are replaced by VPl. VPl is the product of an alternative transcriptional splicing event that places an additional 153 amino acids at the N terminus [15]. No evidence for VPl was observed in the crystal structure [40]. 

Identification of T-cell epitopes has revealed several alternative mechanisms by which MHC class I-restricted peptides are generated for T-cell recognition. The processing of these epitopes can be modulated at transcription/splicing, translation and protein degradation levels. 

P63 This molecule is a member of the p53 family of proteins it exists as multiple protein variants, which are a result of alternative transcript splicing events. The relative concentrations of these protein variants affect the expression and functionality of wild-type p63 and p53 proteins. The p63 protein is expressed in a nuclear pattern in various myoepithelia and is present in the basal layer of squamous epithelium. ... 

For several reasons, the interphase chromosome scaffold remains the most controversial of karyoskeletal elements. For example, it is currently uncertain whether the scaffold is one contiguous element, several discrete elements, or an in vitro artifact without biological significance. Nevertheless, roles for the scaffold in DNA replication, transcription, splicing, and (perhaps) mRNA export have been proposed. In the current context, it is critical to distinguish the interphase scaffold, referred to herein, from the mitotic or metaphase scaffold, studied extensively by others [for a review, see Saitoh and Laemmli (1993) and references therein]. 

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