Total Globulin Fraction of Blood

The average composition of the nonalbumin fraction of blood serum is shown in Table 2. It will be seen that, if we abide by the original definition 

Main Components op Normal Total Globulin of Human Blood Serum 

Since 1905 when Hardy (H4) first observed separation of serum proteins by electrophoresis, there have been repeated suggestions that the plasma proteins are not independent components in vivo that there exist molecules 

Estimation of globulins subdivided by paper electrophoresis into the various o, and 7 types is of very limited value in clinical medicine (07). Furthermore, information obtained by it can usually be obtained with greater ease and accuracy by simple chemical and clinical tests (07). Sera from patients that might be expected to have abnormal proteins give no characteristic patterns that can be distinguished from normal controls, even when starch-gel electrophoresis is used (B6). 

It has been suggested that a statistical measure of the electrophoretic lack of homogeneity ( entropy ) of the serum globulins might allow pathological processes to be further differentiated (HIO). This mathematical device seems unlikely to be of real value, because paper electrophoresis is an arbitary process dependent chiefly on one physical parameter, namely, molecular charge. 

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Albumin and Total Globulin Fractions of Blood Derek Watson... 

After 4 1/2 hours 1.0 ml. blood contained 1.0 x 105 counts/min. Electrophoretic separation of plasma showed 77% of Cu associated with albumin fractions, 1 to 3% in each a-globulin fraction (total 10%), 1 to 3% in each -globulin fraction (total 10%), and about 3% in y-globulins... 

In the above methods, the separated albumin (or whole serum if total protein is to be determined) is reacted with excess copper in approximately 1 N alkali. A macromethod is also available which utilizes the capacity of albumin to react with copper(II) in a mole ratio of 1 1 (K32). This is an amperometric titration of blood serum (1 ml) in 0.1 M ammoniacal am-moniiun nitrate pH 9.2 with CuS04 (4.8 X 10 M). Under these conditions human y-globulin did not react, so that the fair agreement by this method with results obtained by a biuret method after sulfite fractionation (K32) is probably the result of a balance of errors. In view of the interest in the small fraction of albumin-boimd copper in hepatolenticular degeneration, and the paucity of information on the unique binding reaction presumably with N-terminal aspartyl residues, further investigation would be valuable. 

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