Toluidine blue residues

Cell walls in the necrotic tissue of these wounds were browned. Staining with diazotized Q-tolidine and toluidine blue confirmed the polypheno-lic nature of these brown depositions, which may have resulted from the polymerization of the stilbenes present in large quantities in spruce bark. Phenolic residues were deposited on the walls of certain cells internal to the necrotic tissues by 10 days after wounding. By 36 days these cells had become thick-walled. The precise nature of substances responsible for this thickening has not been determined, variable responses being obtained with histochemical tests for lignin (cf. Table I). Suberin was detectable in cells immediately underlying the thick walled cells, which corresponded to the... 

Mild, selective oxidation of the sialic acid side-chain, followed by the addition of bisulfite and Toluidine Blue has been used for topo-optical staining of sialoglycoconjugates in erythrocyte and lymphocyte membranes. This method, which is based on a polarization-microscope technique, pennits not only specific, and very sensitive, light-microscope staining of sialic acid residues on cell surfaces but also the study of the spatial arrangement of sialoglycoconjugate molecules on cell surfaces.192,197-198... 

Figure 2.11 Electrophoresis of PolyPs with average chain lengths of (a) 15, (b) 25, (c)45 (Sigma) and (d) 188 (Monsanto) phosphate residues in 20 % polyacrylamide gel (PAAG) with 7 M urea staining was achieved by using toluidine blue (Pestov et al., 2003).

Materials. Dodecylpyridinium bromide was synthesized by treating fractionally distilled 1-dodecane bromide in dry pyridine for 12 hr. The crude surfactant was recrystallized twice from acetone followed by decolorization with active charcoal in methanol solution. The resulting white crystal is a monohydrate of DoPBr. The critical micelle concentration (CMC) in aqueous solution as determined by electric conductivity method is 17.4 mM at 30°C in agreement with literature. Calf thymus DNA (sodium salt, SIGMA) was used as received. Residual (nucleotide) concentration was determined by a colloid titration using poly(potassium vinyl sulfate) as a titrant and Toluidine Blue as an indicator. 4 Propionyl- a-cyclodextrin (prop- a-CD) used as a neutral carrier was prepared by esterification of a-cyclodextrin (Tokyo Kasei Co.) with propionic anhydride in dry pyridine at room temperature for 12 hr. The reaction mixture was poured onto ice to obtain a gummy product which was then dissolved in acetone and precipitated in cold water. The dissolution-precipitation was repeated three times. The hydrophobicized oc-CD is a white powder. 

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