Tobacco mutant

Separation and Assay. H.p.l.c. methods have been described for the determination of chloroplast pigments,phytoplankton pigments,and provitamin A carotenoids in tomatoes.Other chromatographic procedures have been devised for the separation of Capsicum carotenoids and chloroplast pigments from tobacco mutants, " and methods for the high-speed video-densitometric determination of carotenoids separated by t.l.c., and for the dual assay of carotenoids and vitamin A in human liver have been reported. H.p.l.c. has been used for the separation of cis-trans-isomers of retinal, retinol and retinyl... 

Gondet, L., Bronner, R. and Benveniste, P. (1994) Regulation of sterol content in membranes by subcellular compartmentation of steol-esters accumulating in a sterol-overproducing tobacco mutant. Plant Physiol., 105, 509-18. 

Gondet, L., Weber, T., Maillot-Vernier, R, Benveniste, R. and Bach, T.J. (1992) Regulatory role of microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase in a tobacco mutant that overproduces sterols. Biochem. Biophys. Res. Commun., 186, 888-93. 

Bouvier-Nave, R and Benveniste, P. (1995) Sterol acyl transferase and steryl ester hydrolase activities in a tobacco mutant which overproduces sterols. Plant Sci, 110, 11-9. 

Revertant of no-active RuBisCO tobacco mutant, Sp25, obtained by chloroplast transformation method using microprojectile bombardment... 

Our preliminary results Indicate that, like the chlorsulfuron-reslstant tobacco mutant, the A. thallana line GH50 has... 

Fig. 1 Comparison of RuBP carboxylase/oxygenase of tabacum var. JVJB with that of the tobacco mutant Su/su, Su/su var. Aurea, Spinacia oleracea by tandem crossed immuno electrophoresis.

LIPID COMPOSITION OF THYLAKOID MEMBRANES FROM TOBACCO MUTANT CHLOROPLASTS EXHIBITING EITHER ONLY PHOTOSYSTEM I OR PHOTOSYSTEM I AND II ACTIVITY... 

Serological studies have shown that the lipid distribution in the lamellar system of chloroplasts shows a lateral and transversal asymmetry (Radunz, 1980). We have analysed the lipid composition of chloroplasts from the variegated tobacco mutant Nicotiana tabacum NC 95. 

Table 1 Per Cent of Lipids Referred to the Total Lipid Content of Chloroplasts from Green (Chl ) and Yellow-Green (Chly ) Leaf Areas and of Photosystem II Particles (PS II), Reaction Center Complexes (RCC) and Light-Harvesting Complexes (LHC) from Green Leaf Areas of the Tobacco Mutant Nicotiana tabacum NC 95... 

Lipid composition of photosystem I and II in the tobacco mutant Nicotiana tabacum NC 95 Z. Naturforsch. 43c, 423-430... 

STUDY ON THE LIPID COMPOSITION OF CHLOROPLASTS OF THE TOBACCO MUTANT N. TABACUM VAR. XANTHI EXHIBITING AN INCREASED PHOTOSYSTEM I ACTIVITY... 

A substantial difference between chloroplasts of different photosynthetic activities appears to exist with respect to the phospholipid content. Whereas in normally developed chloroplasts of green leaf areas the phospholipid content is approximately 7%, this content increases in chloroplasts with exclusively photosystem I activity to 14% and increases further in chloroplasts with increased photosystem I activity to 28% of the total lipid content whereas it appears that the ratio of phosphatidylglycerol to phosphatidylcholine and tp phosphatidylinositol is the same in the two types of chloroplasts of the plastome mutant. Chloroplasts of the tobacco mutant NC 95 with only photosystem I activity seem to contain besides phosphat i dyl i nosi tol essentiall-y only phosphatidylcholine as the main component. 

De Jong, D.W. and Woodhef, W.C., High speed, low pressure hquid chromatography of chloroplast pigments from tobacco mutants, J. Agric. Food Chem., 26, 1281, 1978. 

I wish to thank Dr. G. Retzlaff, BASF AG., D-6703 Limburgerhof, for providing sethoxydim, Mr. Guido Schmuck, Karlsruhe for the data on the tobacco mutant (Table 2, Fig. 2), Mrss. Ursula Prenzel and Inge Schurer, Karlsruhe, for excellent assistance and Mr. Philip Jackson, Karlsruhe, for checking the English text. 

Maillot-Vernier P, Gondet L, Schaller H, Benveniste P, Belliard G. Genetic study and further biochemical characterization of a tobacco mutant that overproduces sterols. Mol Gen Genet, 1991 231 33-40. 

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