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Chloroplast Transformation Methods

Revertant of no-active RuBisCO tobacco mutant, Sp25, obtained by chloroplast transformation method using microprojectile bombardment... [Pg.609]

Cell culture-based methods suitable for obtaining chloroplast-encoded herbicide resistance in crops will be reviewed. These will Include mutant selection in cultured cells, transfer of chloroplasts via protoplast fusion, and selection of recombinant chloroplast genomes. Examples will be limited to resistance to triazine herbicides. Chloroplast transformation involving introduction of novel DNA into chloroplasts, will not be covered. Preliminary data indicate that chloroplast transformation may be feasible (4) but have not been confirmed. The genetics, physiology... [Pg.115]

Transformation of protoplasts by PEG has been demonstrated to work well for chloroplasts as well as plant nuclei. Since chloroplasts tend to be appressed to the cell membranes in protoplasts, they can be penetrated easily by this method. However, this approach is technically more demanding than biolistic delivery and involves a considerably longer period of time for the selection process (Heifetz, 2000). [Pg.62]

Particle bombardment remains the most effective method for transforming the chloroplast of C. reinhardtii, and was first demonstrated in the 1980s [64]. [Pg.610]


See other pages where Chloroplast Transformation Methods is mentioned: [Pg.82]    [Pg.610]    [Pg.82]    [Pg.610]    [Pg.7]    [Pg.131]    [Pg.142]    [Pg.609]    [Pg.2196]    [Pg.442]    [Pg.251]    [Pg.119]    [Pg.98]    [Pg.36]    [Pg.123]    [Pg.36]    [Pg.606]    [Pg.606]    [Pg.570]    [Pg.386]   
See also in sourсe #XX -- [ Pg.609 ]




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